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Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration

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Peripheral Nerve Tissue Engineering and Regeneration

Part of the book series: Reference Series in Biomedical Engineering ((TIENRE))

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Abstract

In peripheral nerve studies, histological analyses represent one of the most widely used and informative quality controls to demonstrate nerve tissue regeneration. These analyses require several technical procedures, and a basic knowledge of nerve histology and regeneration is needed for the correct interpretation of the histological information obtained from the experimental studies. For this reason, the aim of this chapter is to review the basic nerve histology and regeneration, as well as the technical procedures and stainings available for the assessment of nerve tissue regeneration. In this sense, some concepts and technical details of the nerve tissue fixation and processing for light and electron microscopy are comprehensively discussed. In relation to histological staining, they were reviewed according to their application in the assessment of nerve tissue regeneration, stromal remodeling, and host response following nerve repair and regeneration. Finally, the importance and main advantages offered by the quantitative analyses of nerve regeneration, mainly focused on the semithin and ultrastructural analyses, were reported.

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Acknowledgments

This research was funded by the Spanish “Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III),” Grants N° FIS PI17-0393, FIS PI20-0318 co-financed by the “Fondo Europeo de Desarrollo Regional ERDF-FEDER European Union”; Grant N° P18-RT-5059 by “Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020), Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Junta de Andalucía, España”; Grant N° A-CTS-498-UGR18 by “Programa Operativo FEDER Andalucía 2014-2020, Universidad de Granada, Junta de Andalucía, España,” co-funded by ERDF-FEDER, the European Union; and Grant Nº INTRAIBS-2020-08 from Instituto de Investigación Biosanitaria ibs.GRANADA. The authors are grateful to Prof. Dr. Ariane Ruyffelaert (University of Granada) for her proofreading service.

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Author notes

  1. Jesús Chato-Astrain and Óscar D. García-García contributed equally to this chapter.

Authors and Affiliations

  1. Department of Histology & Tissue Engineering Group, University of Granada, Granada, Spain

    Jesús Chato-Astrain, Óscar D. García-García, Fernando Campos, David Sánchez-Porras & Víctor Carriel

  2. Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain

    Jesús Chato-Astrain, Óscar D. García-García, Fernando Campos, David Sánchez-Porras & Víctor Carriel

  3. Av. de la Investigación n°11, Facultad de Medicina, Torre A, Departamento de Histología, Universidad de Granada, Granada, Spain

    Víctor Carriel

Authors
  1. Jesús Chato-Astrain
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  2. Óscar D. García-García
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  3. Fernando Campos
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  4. David Sánchez-Porras
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  5. Víctor Carriel
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Correspondence to Víctor Carriel .

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Editors and Affiliations

  1. Pharmacology, UCL School of Pharmacy, University College London Pharmacology, UCL School of Pharmacy, London, UK

    James Phillips

  2. Experimentelle u Klinische Traumatologie, Ludwig Boltzmann Institut für, Wien, Wien, Austria

    David Hercher

  3. Trauma Surgery, AUVA Unfallkrankenhaus Lorenz Böhler, Wien, Wien, Austria

    Thomas Hausner

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Chato-Astrain, J., García-García, Ó.D., Campos, F., Sánchez-Porras, D., Carriel, V. (2021). Basic Nerve Histology and Histological Analyses Following Peripheral Nerve Repair and Regeneration. In: Phillips, J., Hercher, D., Hausner, T. (eds) Peripheral Nerve Tissue Engineering and Regeneration. Reference Series in Biomedical Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-030-06217-0_14-1

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  • DOI: https://doi.org/10.1007/978-3-030-06217-0_14-1

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