Encyclopedia of Sustainability Science and Technology

Living Edition
| Editors: Robert A. Meyers

Crop Responses to Nitrogen

  • Gilles Lemaire
Living reference work entry

Later version available View entry history

DOI: https://doi.org/10.1007/978-1-4939-2493-6_385-3


A prerequisite for the analysis of crop responses to nitrogen (N) is the determination of the plant nitrogen content and repartition. How much N is incorporated within plants and crops? Within which plant tissue? For which physiological function? Thus, according to the answers to these questions, it is possible to determine a critical plant nitrogen status as the minimum plant N concentration that allows the maximum plant (or crop) growth rate. It has been demonstrated that this critical plant N concentration decreases as plant grows as the result of an ontogenetic plant architecture development leading to a dilution of Ncompounds within increasing proportion of free-N compounds as plant gets bigger. This N dilution process can be formulated through a negative power relationship between plant N concentration and crop mass. This critical N dilution curve allows the discrimination of situations of N deficiency (below the curve) and situations of N luxury consumption (above...


Leaf Area Index Tall Fescue Uptake Capacity Supply Condition Leaf Area Ratio 
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Primary Literature

  1. 1.
    Angus JF (2001) Nitrogen demand and supply in Australian agriculture. Aust J Exp Agric 41:277–288CrossRefGoogle Scholar
  2. 2.
    Eikhout B, Bouwman AF, Zeijts VH (2006) The role of nitrogen in world food production and food sustainability. Agric Ecosyst Environ 116:4–14CrossRefGoogle Scholar
  3. 3.
    London JG (2005) Nitrogen study fertilizes fears of pollution. Nature 433:791CrossRefGoogle Scholar
  4. 4.
    Beman JM, Arrigo K, Matson PM (2005) Agricultural runoff fuels large phytoplankton blooms in vulnerable areas of the ocean. Nature 434:211–214CrossRefGoogle Scholar
  5. 5.
    Ramos C (1996) Effect of agricultural practices on the nitrogen losses in environment. In: Rodriguez-Barrueco C (ed) Fertilizer and environment. Kluwer, Dordrecht, pp 335–361Google Scholar
  6. 6.
    Stulen I, Perez-Soba M, De kok LJ, Van Der Eerden L (1998) Impact of gaseous nitrogen deposition on plant functioning. New Phytol 139:61–70CrossRefGoogle Scholar
  7. 7.
    Cassman KG (2007) Climate change, biofuels, and global food security. Environ Res Lett 2:11–12CrossRefGoogle Scholar
  8. 8.
    Addiscott TM, Withmore AP, Powlson DS (1991) Farming, fertilizers and nitrate problem. CAB International, Wallingford, p 170Google Scholar
  9. 9.
    Hirel B, Lemaire G (2005) From agronomy and ecophysiology to molecular genetics for improving nitrogen use efficiency in crops. J Crop Improv 15:213–257CrossRefGoogle Scholar
  10. 10.
    Evans JR (1983) Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum L.). Plant Physiol 72:297–302CrossRefGoogle Scholar
  11. 11.
    Lawlor DW (1995) Photosynthesis, productivity and environment. J Exp Bot 46:1449–1461CrossRefGoogle Scholar
  12. 12.
    Lawlor DW, Konturri M, Young AT (1989) Photosynthesis by flag leaves of wheat in relation to protein, ribulose biphosphate carboxylase activity and nitrogen supply. J Exp Bot 40:43–52CrossRefGoogle Scholar
  13. 13.
    Werger MJA (1991) Leaf nitrogen distribution and whole canopy photosynthetic carbon gain in herbaceous stands. Vegetatio 97:11–20Google Scholar
  14. 14.
    Nakano HM, Mae T (1997) The effects of elevated partial pressure of CO2 on the relationship between photosynthetic capacity and N content in rice leaves. Plant Physiol 115:191–198Google Scholar
  15. 15.
    Makino A, Mae T, Ohira K (1988) Differences between wheat and rice in the enzymic properties of ribulose-1, 5-biphosphate carboxylase/oxygenase and the relationship to photosynthetic gas exchange. Planta 174:30–38CrossRefGoogle Scholar
  16. 16.
    Theobald JC, Mitchell RAC, Parry MAJ, Lawlor DW (1998) Estimating the excess investment in ribulose-1, 5-bisphophate carboxylase/oxygenase in leaves of spring wheat grown under elevated CO2. Plant Physiol 118:945–955CrossRefGoogle Scholar
  17. 17.
    Sage RF (1987) The nitrogen use efficiency of C3 and C4 plants. I- Leaf nitrogen, growth, and biomass partitioning in Chenopodium album (L.) and Amaranthus retroflexus (L.). Plant Physiol 84:954–958CrossRefGoogle Scholar
  18. 18.
    Lawlor DW, Boyle FA, Keys AJ, Kendall AC, Young AT (1988) Nitrate nutrition and temperature effects on wheat: a synthesis of plant growth and nitrogen uptake in relation to metabolic and physiological processes. J Exp Bot 39:329–343CrossRefGoogle Scholar
  19. 19.
    Millard P (1988) The accumulation and storage of nitrogen by herbaceous plants. Plant Cell Environ 11:1–8CrossRefGoogle Scholar
  20. 20.
    Avice JC, Ourry A, Lemaire G, Volenec JJ, Boucaud J (1997) Root protein and vegetative storage protein are key organic nutrients for alfalfa shoot regrowth. Crop Sci 37:1187–1193CrossRefGoogle Scholar
  21. 21.
    Ourry A, McDuff JH, Ougham H (1996) The relationship between mobilisation of N reserves and changes in translatable messages following defoliation in Lolium temulentum L. and Lolium perenne L. J Exp Bot 47:739–747CrossRefGoogle Scholar
  22. 22.
    Lemaire G, Khaity M, Onillon B, Allirand JM, Chartier M, Gosse G (1992) Dynamics of accumulation and partitioning of N in leaves, stems and roots of lucerne in dense canopy. Ann Bot 70:429–435Google Scholar
  23. 23.
    Grindlay DJC, Sylvester-Bradley R, Scott RK (1995) The relationship between canopy green area and nitrogen in the shoot. In: Lemaire G, Burns IG (eds) Diagnostic procedures for crop N management. INRA Publication, Paris, pp 53–60. INRA-Editions, Collection “Les Colloques”Google Scholar
  24. 24.
    Lemaire G, van Oosterom E, Jeuffroz MH, Gastal F, Massignan A (2008) Crop species present different qualitative types of response to N deficiency during their vegetative growth. Field Crop Res 105:253–265CrossRefGoogle Scholar
  25. 25.
    McAdam JW, Volenec JJ, Nelson CJ (1989) Effects of nitrogen on mesophyll cell division and epidermal cell elongation in tall fescue leaf blades. Plant Physiol 89:549–556CrossRefGoogle Scholar
  26. 26.
    Gastal F, Nelson CJ (1994) Nitrogen use within growing leaf blade of tall fescue. Plant Physiol 105:191–197Google Scholar
  27. 27.
    Fricke W, McDonald AJS, Matson-Djos L (1997) Why do leaves and leaf cells of N-limited barley elongate at reduced rates? Planta 202:522–530CrossRefGoogle Scholar
  28. 28.
    Uhart SA, Andrade FH (1995) Nitrogen deficiency on maize. I – effects on crop growth, development, dry matter partitioning and kernel set. Crop Sci 35:1376–1383CrossRefGoogle Scholar
  29. 29.
    Desmotes-Mainard S, Jeuffroy MH, Robin S (1999) Spike dry matter and nitrogen accumulation before anthesis in wheat as affected by nitrogen fertilizer: relationship to kernel per spike. Field Crop Res 64:249–259CrossRefGoogle Scholar
  30. 30.
    Mae T (1997) Physiological nitrogen efficiency in rice: nitrogen utilization, photosynthesis, and yield potential. In: Ando T (ed) Plant nutrition for sustainable food production and environment. Kluwer, Dordrecht, pp 51–60CrossRefGoogle Scholar
  31. 31.
    Lemaire G, Avice JC, Kim TH, Ourry A (2005) Development changes in shoot N dynamics of lucerne in relation to leaf growth dynamics as a function of plant density and hierarchical position within the canopy. J Exp Bot 56:935–943CrossRefGoogle Scholar
  32. 32.
    Lemaire G, Gastal F (2009) Quantifying crop responses to nitrogen deficiency and avenues to improve nitrogen use efficiency. In: Sadras VO, Calderini DF (eds) Crop physiology: applications for genetic improvement and agronomy. Academic/Elsevier, Amsterdam, pp 171–199CrossRefGoogle Scholar
  33. 33.
    Greenwood DJ, Lemaire G, Gosse G, Cruz P, Draycott A, Neeteson JJ (1990) Decline in percentage N of C3 and C4 crops with increasing plant mass. Ann Bot 66:425–436Google Scholar
  34. 34.
    Lemaire G, Jeuffroy MH, Gastal F (2008) Diagnosis tool for plant and crop N status in vegetative stage. Theory and practices for crop N management. Eur J Agron 28:614–624CrossRefGoogle Scholar
  35. 35.
    Lemaire G, Gastal F (1997) N uptake and distribution in plant canopies. In: Lemaire G (ed) Diagnosis on the nitrogen status in crops. Springer, Heidelberg, pp 3–43CrossRefGoogle Scholar
  36. 36.
    Justes E, Mary B, Meynard JM, Machet JM, Thellier-Huché L (1994) Determination of a critical nitrogen dilution curve for winter wheat crops. Ann Bot 74:397–407CrossRefGoogle Scholar
  37. 37.
    Lemaire G, van Oosterom E, Sheehy J, Jeuffroy MH, Massignan A, Rossato L (2007) Is crop demand closely related to dry matter accumulation of leaf area expansion during vegetative growth? Field Crop Res 100:91–106CrossRefGoogle Scholar
  38. 38.
    Gastal F, Lemaire G (2002) N uptake and distribution in crops: an agronomical and ecophysiological perspective. J Exp Bot 53:789–799CrossRefGoogle Scholar
  39. 39.
    Lemaire G, Salette J (1984) Relation entre dynamique de croissance et dynamique de prélèvement d’azote par un peuplement de graminées fourragères. 1- Etude de l′effet du milieu. Agronomie 4:423–430CrossRefGoogle Scholar
  40. 40.
    Lemaire G, Salette J (1984) Relation entre dynamique de croissance et dynamique de prélèvement d'azote par un peuplement de graminées fourragères. 2- Etude de la variabilité entre génotypes. Agronomie 4:431–436CrossRefGoogle Scholar
  41. 41.
    Lemaire G, Cruz P, Gosse G, Chartier M (1985) Etude des relations entre la dynamique de prélèvement d'azote et la dynamique de croissance en matière sèche d'un peuplement de luzerne. Agronomie 5:685–692CrossRefGoogle Scholar
  42. 42.
    Ney B, Doré T, Sagan M (1997) The nitrogen requirement of major agricultural crops: grain legumes. In: Lemaire G (ed) Diagnosis of the nitrogen status in crops. Springer, Heidelberg, pp 107–117CrossRefGoogle Scholar
  43. 43.
    Colnenne C, Meynard JM, Reau R, Justes E, Merrien A (1998) Determination of a critical nitrogen dilution curve for winter oilseed rape. Ann Bot 81:311–317CrossRefGoogle Scholar
  44. 44.
    Sheehy JE, Dionara MJA, Mitchell PL, Peng S, Cassman KG, Lemaire G, Williams RL (1998) Critical concentrations: implications for high-yielding rice (Oryza sativa, L.) cultivars in tropics. Field Crop Res 59:31–41CrossRefGoogle Scholar
  45. 45.
    Tei F, Benincasa P, Guidici M (2002) Critical nitrogen concentration in processing tomato. Eur J Agron 18:45–56CrossRefGoogle Scholar
  46. 46.
    Plénet D, Lemaire G (1999) Relationships between dynamics of nitrogen uptake and dry matter accumulation in maize crops. Determination of critical N concentration. Plant Soil 216:65–82CrossRefGoogle Scholar
  47. 47.
    Plénet D, Cruz P (1997) The nitrogen requirement for major agricultural crops: maize and sorghum. In: Lemaire G (ed) Diagnosis of the nitrogen status in crops. Springer, Heidelberg, pp 93–106CrossRefGoogle Scholar
  48. 48.
    Duru M, Lemaire G, Cruz P (1997) The nitrogen requirement for major agricultural crops: grasslands. In: Lemaire G (ed) Diagnosis of nitrogen status in crops. Springer, Heidelberg, pp 56–72Google Scholar
  49. 49.
    Hardwick RC (1987) The nitrogen content of plants and the self thinning rule in plant ecology: a test of the core-skin hypothesis. Ann Bot 60:439–446Google Scholar
  50. 50.
    Ismande J, Touraine B (1994) N demand and regulation of nitrate uptake. Plant Physiol 105:3–7Google Scholar
  51. 51.
    Lejay L, Tillard P, Petit M, Olive FD, Filleur S, Daniel-Vedele F, Gojon A (1999) Molecular and functional regulation of two NO3- uptake systems by N and C status of Arabidopsis plants. Plant J 18:509–519CrossRefGoogle Scholar
  52. 52.
    Forde BG (2002) The role of long-distance signaling in plant response to nitrate and other nutrients. J Exp Bot 53:39–43CrossRefGoogle Scholar
  53. 53.
    Glass ADM, Britto DT, Kaiser BN, Kinghorn JR, Kronzucker HJ, Kumar A, Okamoto M, Rwat S, Siddiqi MY, Unkless SE, Vidmar J (2002) The regulation of nitrate and ammonium transport systems in plants. J Exp Bot 53:855–864CrossRefGoogle Scholar
  54. 54.
    Devienne-Barret F, Justes E, Machet JM, Mary B (2000) Integrated control of nitrate uptake by crop growth rate and soil nitrate availability under field conditions. Ann Bot 86:995–1005CrossRefGoogle Scholar
  55. 55.
    Lemaire G, Meynard JM (1997) Use of the nitrogen nutrition index for the analysis of agronomical data. In: Lemaire G (ed) Diagnosis of the nitrogen status in crops. Springer, Heidelberg, pp 45–56CrossRefGoogle Scholar
  56. 56.
    Angus JF, Moncur MW (1985) Models of growth and development of wheat in relation to plant nitrogen. Aust J Agr Res 36:537–544CrossRefGoogle Scholar
  57. 57.
    Jeuffroy MH, Bouchard C (1999) Intensity and duration of nitrogen deficiency on wheat grain number. Crop Sci 39:1385–1393CrossRefGoogle Scholar
  58. 58.
    Lemaire G, Plénet D, Grindlay D (1997) Leaf N content as an indicator of crop N nutrition. In: Lemaire G (ed) Diagnosis on the nitrogen status in crops. Springer, Heidelberg, pp 189–199CrossRefGoogle Scholar
  59. 59.
    Farrugia A, Gastal F, Scholefield D (2004) Assessment of nitrogen status of grasslands. Grass Forage Sci 59:113–120CrossRefGoogle Scholar
  60. 60.
    Ziadi N, Bélanger G, Gastal F, Claesses A, Lemaire G, Tremblay N (2009) Leaf nitrogen concentration as an indicator of corn nitrogen status. Agron J 101:947–957CrossRefGoogle Scholar
  61. 61.
    Alam MM, Ladha JK, Rahman Khan S, Harun-Ur-Rashid Khan AH, Buresh RJ (2005) Leaf color chart for managing nitrogen fertilizer in lowland rice in Bangladesh. Agron J 97:949–959CrossRefGoogle Scholar
  62. 62.
    Peng S, Garcia FV, Laza RC, Sanico AL, Visperas RM, Cassman KG (1996) Increased N-use efficiency using a chlorophyll meter on high-yielding irrigated rice. Field Crop Res 47:243–252CrossRefGoogle Scholar
  63. 63.
    Feibo W, Lianghuan W, Fuha X (1998) Chlorophyll meter to predict nitrogen side dress requirements for short-season cotton. Field Crop Res 56:309–314CrossRefGoogle Scholar
  64. 64.
    Le Bail M, Jeuffroy MH, Bouchard C, Barbotin A (2005) Is it possible to forecast the grain quality and yield of different varieties of winter wheat from minolta SPAD meter measurements? Eur J Agron 23:379–391CrossRefGoogle Scholar
  65. 65.
    Houlès V, Guérif M, Mary B (2007) Elaboration of a nutrition indicator for winter wheat blades on leaf area index and chlorophyll meter content for making nitrogen recommendations. Eur J Agron 27:1–11CrossRefGoogle Scholar
  66. 66.
    Monteith JL (1972) Solar radiation and productivity in tropical ecosystems. J Appl Ecol 9:747–766CrossRefGoogle Scholar
  67. 67.
    Bélanger G, Gastal F, Lemaire G (1992) Growth analysis of a tall fescue sward fertilized with different rates of nitrogen. Crop Sci 6:1371–1376CrossRefGoogle Scholar
  68. 68.
    Trapani N, Hall AJ (1996) Effects of leaf position and nitrogen supply on the expansion of leaves of field-grown sunflower. Plant Soil 184:331–340CrossRefGoogle Scholar
  69. 69.
    Muchow RC, Davis R (1998) Effect of nitrogen supply on the comparative productivity of maize and sorghum in a semi-arid tropical environment. II- radiation interception and biomass accumulation. Field Crop Res 18:17–30CrossRefGoogle Scholar
  70. 70.
    Bélanger G, Gastal F, Warembourg F (1994) Carbon balance on tall fescue: effects of nitrogen and growing season. Ann Bot 74:653–659CrossRefGoogle Scholar
  71. 71.
    Robson MJ, Parsons AJ (1978) Nitrogen deficiency in small closed communities of S24 ryegrass. I-Photosynthesis, respiration, dry matter production and partition. Ann Bot 42:1185–1197Google Scholar
  72. 72.
    Jarvis SC, McDuff JH (1989) Nitrate nutrition of grasses from steady state supplies in flowing solution culture following nitrate deprivation and/or defoliation. J Exp Bot 40:695–975CrossRefGoogle Scholar
  73. 73.
    Gastal F, Bélanger G, Lemaire G (1992) A model of the leaf extension rate of tall fescue in response to nitrogen and temperature. Ann Bot 70:437–442Google Scholar
  74. 74.
    Sinclair TR, Horie T (1989) Leaf nitrogen, photosynthesis, and crop radiation use efficiency: a review. Crop Sci 29:90–98CrossRefGoogle Scholar
  75. 75.
    Grindlay DJC (1997) Towards an explanation of crop nitrogen demand based on leaf nitrogen per unit leaf area. J Sci Food Agric 63:116–123Google Scholar
  76. 76.
    Van Keulen H, Goudrian J, Seligman NG (1989) Modelling the effects of nitrogen on canopy development and crop growth. In: Rusell G, Marshall LB, Jarvis PG (eds) Plant canopies: their growth, form and function. Cambridge University Press, Cambridge, pp 83–104CrossRefGoogle Scholar
  77. 77.
    Gastal F, Bélanger G (1993) The effects of nitrogen fertilization and the growing season on photosynthesis of field-grown tall fescue canopies. Ann Bot 72:401–408CrossRefGoogle Scholar
  78. 78.
    Connor DJ, Hall AJ, Sadras VO (1993) Effects of nitrogen content on the photosynthetic characteristics of sunflower crops during grain filling. Aust J Plant Physiol 20:251–263CrossRefGoogle Scholar
  79. 79.
    Fischer RA (1993) Irrigated spring wheat and timing and amount of nitrogen fertilizer. II-Physiology of grain yield response. Field Crop Res 33:57–80CrossRefGoogle Scholar
  80. 80.
    Martre P, Porter JR, Jamieson PD, Triboï E (2003) Modelling grain nitrogen accumulation and protein composition to understand the sink/source regulations of nitrogen utilization in wheat. Plant Physiol 133:1959–1967CrossRefGoogle Scholar
  81. 81.
    Desmotes-Mainard S, Jeuffroy MH (2004) Effects of nitrogen and radiation on dry matter and nitrogen accumulation in spike of winter wheat. Field Crop Res 87:221–233CrossRefGoogle Scholar
  82. 82.
    Dreccer MF (2005) Nitrogen use at the leaf and canopy level: a framework to improve N use efficiency. J Crop Improv 15:97–125CrossRefGoogle Scholar
  83. 83.
    Borell A, Hammer GL (2000) Nitrogen dynamics and the physiological basis for stay-green in sorghum. Crop Sci 40:1295–1307CrossRefGoogle Scholar
  84. 84.
    Mi C, Liu J, Chen F, Zhang F, Cui Z, Liu X (2003) Nitrogen uptake and remobilization in maize hybrids differing in leaf senescence. J Plant Nutr 26:447–459CrossRefGoogle Scholar
  85. 85.
    Moll RH, Kamprath EJ, Jackson WA (1982) Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron J 74:562–564CrossRefGoogle Scholar
  86. 86.
    Borell A, Garside AL, Fukaï S, Reid DJ (1998) Season nitrogen rate and plant type affect nitrogen uptake and nitrogen use efficiency in rice. Aust J Agr Res 49:829–843CrossRefGoogle Scholar
  87. 87.
    Le Gouis J, Béghin D, Heumez E, Pluchard P (2000) Genetic differences for nitrogen uptake and nitrogen utilization efficiencies in winter wheat. Eur J Agron 12:163–173CrossRefGoogle Scholar
  88. 88.
    Bertin P, Gallais A (2001) Physiological and genetic basis of nitrogen use efficiency on maize. Maydica 46:53–68Google Scholar
  89. 89.
    Lemaire G, Charrier X, Hébert Y (1996) Nitrogen uptake capacities of maize and sorghum crops in different nitrogen and water supply conditions. Agronomie 16:231–246CrossRefGoogle Scholar
  90. 90.
    Lemaire G, Recous S, Mary B (2004) Managing residues and nitrogen in intensive cropping systems. New understandings for efficient recovery by crops. In: Proceedings of the 4th international crop science congress, Brisbane, 2004Google Scholar
  91. 91.
    Laperche A, Devienne-Barret F, Maury O, Le Gouis J, Ney B (2007) A simplified conceptual model of carbon and nitrogen functioning for QTL analysis of winter wheat adaptation to nitrogen deficiency. Theor Appl Genet 113:1131–1146CrossRefGoogle Scholar
  92. 92.
    Camus-Kulandaivelu L, Veyreiras JB, Madur D, Combes V, Fourman M, Barraud S, Dubreuil P, Gouesnard B, Manicacci D, Charcosset A (2006) Maize adaptation to temperate climate: relationship between population structure and polymorphism in the Dwarf 8 gene. Genetics 172:2449–2463CrossRefGoogle Scholar
  93. 93.
    Singh U, Ladha JK, Castillo EG, Punzalan G, Tirol-Padre A, Duqueza M (1998) Genotypic variation in nitrogen use efficiency. I-medium and long-duration rice. Field Crop Res 58:35–53CrossRefGoogle Scholar
  94. 94.
    Sadras VO (2006) The N:P stoichiometry of cereal, grain legume and oilseed crops. Field Crop Res 95:13–29CrossRefGoogle Scholar
  95. 95.
    Gastal F, Lemaire G, Durand JL, Louarn G (2009) Quantifying crop responses to nitrogen deficiency and avenues to improve nitrogen use efficiency. In: Sadras VO, Calderini DF (eds) Crop physiology: applications for genetic improvement and agronomy. Second edition. Academic/Elsevier, pp 161–206Google Scholar

Books and Reviews

  1. Sinclair TR (1998) Historical changes in harvest index crop N accumulation. Crop Sci 38:638–643Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.INRALusignanFrance