Synonyms
Quantitative sequencing by synthesis
Definition
Pyrosequencing is a sequencing-by-synthesis method that quantitatively monitors the real-time incorporation of nucleotides using an enzymatic conversion of pyrophosphate into a proportional light signal. Quantitative measures are crucial for applications such as the analysis of DNA methylation patterns, which are intensively studied in various developmental and pathological contexts as well as for bacterial identification and determination of allelic imbalance.
Introduction
While Sanger sequencing has been the “gold standard” for the identification of sequence variants for a long time, pyrosequencing with its improved ability for quantification, decreased limit of detection and accelerated workflow leading to a shorter time to results, has become a valuable alternative notably for many clinical and diagnostic applications. Pyrosequencing is a sequencing-by-synthesis method, where nucleotides are incorporated complementary to a...
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Busato, F., Tost, J. (2015). DNA Methylation Analysis by Pyrosequencing. In: Nelson, K.E. (eds) Encyclopedia of Metagenomics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-7478-5_799
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DOI: https://doi.org/10.1007/978-1-4899-7478-5_799
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