Evaluating Putative Chimeric Sequences from PCR-Amplified Products
The term chimera has its origins in the Greek mythology defining a creature composed of body parts from different living beings. In molecular biology, a chimeric sequence or chimera is a DNA sequence composed of DNA fragments originated from two or more genes or genomes.
Chimeric sequences can be naturally generated during DNA recombination which occurs naturally within a genome or by taking up foreign DNA by an organism. These processes of crossover recombination are of interest in phylogenetic and evolution studies and need to be identified (Posada and Crandall 2002). Nevertheless, chimeras represent a serious problem to be considered when they are generated as artifacts during DNA manipulation and/or analysis.
The author acknowledges funding from the Spanish Ministry of Economy and Competitiveness, project CONSOLIDER CSD2009-00006, which includes participation of Feder funds.
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