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Simultaneous Quantification of Multiple Bacteria

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Encyclopedia of Metagenomics

Synonyms

Composition assessment; Abundance determination

Definition

The term “quantification” derives from the Latin terms quant (meaning “how much”) and facere (meaning “to make”). Quantifying is the act of determining the quantity of, measure. Simultaneous quantification of multiple bacteria stands for medium- to high-throughput detection and abundance determination of a bacterial community.

Introduction

Bacteria are widespread and abundant in the environment and comprise bacterial strains which are pathogenic for plants, animals, or human beings. Historically, the threat represented by pathogenic bacteria lead to the development of cultural-, serological-, and molecular-based methods to identify and possibly quantify the causative agent of an occurring infectious disease. These methods commonly target with high sensitivity and specificity one or few bacterial strains or species, but are not suitable for collecting comprehensive information on a microbial community. Recently, the...

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References

  • Ballarini A, Segata N, Huttenhower C, Jousson O. Simultaneous quantification of multiple bacteria by the bactochip microarray designed to target species-specific marker genes. PLoS One. 2013;8(2):e55764.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Brodie EL, DeSantis TZ, Parker JPM, Zubietta IX, Piceno YM, Andersen GL. Urban aerosols harbor diverse and dynamic bacterial populations. Proc Natl Acad Sci U S A. 2007;104:299–304.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ghosh D, Roy K, Williamson KE, Srinivasiah S, Wommack KE, Radoseevich M. Acyl-homoserine lactones can induce virus production in lysogenic bacteria: an alternative paradigm for prophage induction. Appl Environ Microbiol. 2009;75:7142–52.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hazen TC, Dubinsky EA, DeSantis TZ, Andersen GL, Piceno YM, Singh N, Jansson JK, Probst A, Borglin SE, Fortney JL, Stringfellw WT, Bill M, Conrad ME, Tom LM, Chavarria KL, Alusi TR, Lamendella R, Zhou J, Mason OU. Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science. 2010;330:204–8.

    Article  CAS  PubMed  Google Scholar 

  • Jaing C, Gardner S, McLoughlin K, Mulakken N, Alegria-Hartman M, Banda P, Williams P, Gu P, Wagner M, Manohar C, et al. A functional gene array for detection of bacterial virulence elements. PLoS One. 2008;3(5):e2163.

    Article  PubMed Central  PubMed  Google Scholar 

  • Palacios G, Quan P-L, Jabado O, Conlan S, Hirschberg D, Liu Y. Panmicrobial oligonucleotide array for diagnosis of infectious diseases. Emerg Infect Dis. 2007;13(1):73–81.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Preza D, Olsen I, Willumpsen T, Boches SK, Cotton SL, Grinde B, Paster BJ. Microarray analysis of microflora in root caries of the elderly. Eur J Clin Microbiol Infect Dis. 2009;28(5):509–517.

    Google Scholar 

  • Rajilic-Stojanovic M, Heilig HGHJ, Molenaar D, Kajander K, Surakka A, Smidt H, de Vos WM. Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundance microbiota of young and elderly adults. Environ Microbiol. 2009;11(7):1736–51.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Roh SW, Abell GCJ, Kim K-H, Nam YD, Bae JW. Comparing microarrays and next-generation sequencing technologies for microbial ecology research (review). Trends Biotechnol. 2010; 28(6):291–299.

    Google Scholar 

  • Wu CH, Sercu B, Van De Werfhorst LC, Wong J, DeSantis TZ, Brodie EL, Hazen TC, Holden PA, Andersen GL. Characterization of coastal urban watershed bacterial communities leads to alternative community-based indicators. PLoS One. 2010;5:e11285.

    Article  PubMed Central  PubMed  Google Scholar 

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Correspondence to Annalisa Ballarini .

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© 2015 Springer Science+Business Media New York

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Ballarini, A., Jousson, O. (2015). Simultaneous Quantification of Multiple Bacteria. In: Nelson, K.E. (eds) Encyclopedia of Metagenomics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4899-7478-5_194

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