Encyclopedia of AIDS

Living Edition
| Editors: Thomas J. Hope, Douglas Richman, Mario Stevenson

BAF (BANF1)

  • Xiaolei Zhuang
  • Robert Craigie
Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-9610-6_68-1

Definition

Barrier-to-autointegration factor (BAF or BANF1) is a highly conserved 10-KDa protein in multicellular eukaryotes. It was identified as a factor that blocks integration of Moloney murine leukemia virus DNA into itself (autointegration) in vitro. BAF binds DNA nonspecifically and, because it is a dimer, bridges together DNA molecules. BAF also binds the LEM domain, a domain that is shared among lamina-associated polypeptide 2, emerin, and MAN1. BAF is an essential cellular protein, and knockdown or knockout results in a defect in chromosome segregation during mitosis.

Introduction

A role for barrier-to-autointegration factor (BAF or BANF1) in retroviral DNA integration was first inferred from in vitro experiments with Moloney murine leukemia virus. After entry of a virion into the cytoplasm of a newly infected cell, reverse transcriptionoccurs within the reverse transcription complex, a nucleoprotein complex derived from the core of the infecting virion. The resulting...

Keywords

Total Internal Reflection Fluorescence Microscopy Reverse Transcription Complex Nuclear Envelope Assembly Sucrose Gradient Sedimentation Nuclear Envelope Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Protein Expression LaboratoryNational Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of HealthBethesdaUSA
  2. 2.Laboratory of Molecular BiologyNational Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of HealthBethesdaUSA