Encyclopedia of AIDS

Living Edition
| Editors: Thomas J. Hope, Douglas Richman, Mario Stevenson

HIV-2 Diagnosis and Viral Load Measurements

Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-9610-6_53-1


Detection and/or quantification of the presence of HIV-2 in biological materials, distinguishable from related lentiviruses, either directly as virus-specific nucleic acid or protein or indirectly as immunological responses to infection. Viral load measurements relate specifically to genome quantification and infection dynamics, usually within already diagnosed individuals.


Human immunodeficiency virus type 2 (HIV-2), a member of the Retroviridae, represents a related but distinct group of infections to HIV-1, the global agent of acquired immunodeficiency syndrome (AIDS). While HIV-2 bears similarity to HIV-1, it represents a separate genetic, virologic, and epidemiologic entity of viruses with marked differences in natural history and disease associations (HIV Prevention in the Correctional System). Accurate, reliable diagnosis of HIV-2 infections is important not only from an individual perspective but when considering wider epidemiological and clinical...


Sooty Mangabey Monkey SIVsmm Virus 
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  1. Andersson S, Norrgren H, da Silva Z, Biague A, Bamba S, Kwok S, Christopherson C, Biberfeld G, Albert J. Plasma viral load in HIV-1 and HIV-2 singly and dually infected individuals in Guinea-Bissau, West Africa: significantly lower plasma virus set point in HIV-2 infection than in HIV-1 infection. Arch Intern Med. 2000;160(21):3286–93.PubMedCrossRefGoogle Scholar
  2. Ariyoshi K, Berry NJ, Wilkins A, Ricard D, Aaby P, Naucler A, Ngom PT, Jobe O, Jaffar S, Dias F, Tedder RS, Whittle H. A community-based study of HIV-2 proviral load in a rural village in West Africa. J Infect Dis. 1996;173:245–8.PubMedCrossRefGoogle Scholar
  3. Ariyoshi K, Jaffar S, Alabi AS, Berry N, Schim van der Loeff M, Sabally S, N’Gom PT, Corrah T, Tedder R, Whittle H Plasma RNA viral load predicts the rate of CD4 T cell decline and death in HIV-2-infected patients in West Africa. AIDS. 2000; 14(4):339–44.PubMedCrossRefGoogle Scholar
  4. Berry NJ, Ariyoshi K, Jobe O, Ngom PT, Corrah T, Wilkins A, Whittle H, Tedder R. HIV type 2 proviral load measured by quantitative polymerase chain reaction correlates with CD4+ lymphopaenia in HIV type 2-infected individuals. AIDS Res Hum Ret. 1994;10:1031–7.CrossRefGoogle Scholar
  5. Berry NJ, Ariyoshi K, Jaffar S, Sabally S, Corrah T, Tedder R, Whittle H. Low peripheral blood viral HIV-2 RNA in individuals with high CD4 percentage differentiates HIV-2 from HIV-1 infection. J Hum Virol. 1998;1:457–68.PubMedGoogle Scholar
  6. Berry NJ, Ariyoshi K, Balfe P, Tedder RS, Whittle H. Sequence specificity of the human immunodeficiency virus type 2 (HIV-2) Long Terminal Repeat U3 region in vivo allows subtyping of the principal HIV-2 viral subtypes A and B. AIDS Res Hum Ret. 2001;17:263–7.CrossRefGoogle Scholar
  7. Berry NJ, Jaffar S, Schim van der Loeff M, Ariyoshi K, Harding E, N’Gom P, Dias F, Wilkins A, Ricard D, Aaby P, Tedder R, Whittle H. Low level viraemia and high CD4% predict normal survival of a cohort of HIV-2-infected villagers. AIDS Res Hum Ret. 2002;18:1167–73.CrossRefGoogle Scholar
  8. Berry N, Herrera C, Cranage M. Detection, quantification, and characterisation of HIV/SIV. Methods Mol Biol. 2011;665:133–60.PubMedCrossRefGoogle Scholar
  9. Blaak H, Boers PH, Schutten M, van der Endeñ ME, Osterhaus AD. HIV-2-infected individuals with undetectable plasma viremia carry replication-competent virus in peripheral blood lymphocytes. J AIDS. 2004;36(3):777–82.Google Scholar
  10. Damond F, Benard A, Ruelle J, Alabi A, Kupfer B, Gomes P, Rodes B, Albert J, Boni J, Garson J, Ferns B, Matheron S, Chene G, Brun-Vezinet F. ACHIEV2E collaboration on HIV-2 Infection Study Group. Quality control assessment of HIV-2 viral load quantification assays: results from an international collaboration on HIV-2 infection in 2006. J Clin Microbiol. 2008;46:2088–91.PubMedCrossRefPubMedCentralGoogle Scholar
  11. de Silva T, Cotten M, Rowland-Jones SL. HIV-2: the forgotten AIDS virus. Trends Microbiol. 2008;16(12):588–95.PubMedCrossRefGoogle Scholar
  12. Gottlieb GS, Sow PS, Hawes SE, Ndoye I, Redman M, Coll-Seck AM, Faye-Niang MA, Diop A, Kuypers JM, Critchlow CW, Respess R, Mullins JI, Kiviat NB. Equal plasma viral loads predict a similar rate of CD4+ T cell decline in human immunodeficiency virus (HIV) type 1- and HIV-2-infected individuals from Senegal, West Africa. J Infect Dis. 2002;185(7):905–14.PubMedCrossRefGoogle Scholar
  13. Holmes H, Berry N, Heath A, Morris C. Preparation and evaluation of the 1st international standard for the quantitation of HIV-2 RNA in plasma. J Virol Methods. 2011;175(2):246–52.PubMedCrossRefGoogle Scholar
  14. Lemey P, Pybus OG, Wang B, Saksena NK, Salemi M, Vandamme AM. Tracing the origin and history of the HIV-2 epidemic. Proc Natl Acad Sci U S A. 2003;100(11):6588–92.PubMedCrossRefPubMedCentralGoogle Scholar
  15. MacNeil A, Sarr AD, Sankalé JL, Meloni ST, Mboup S, Kanki P. Direct evidence of lower viral replication rates in vivo in human immunodeficiency virus type 2 (HIV-2) infection than in HIV-1 infection. J Virol. 2007;81(10):5325–30.PubMedCrossRefPubMedCentralGoogle Scholar
  16. Padua E, Jenkins A, Bootman J, Paixo M, Almond N, Berry N. Natural variation of the nef gene in human immunodeficiency virus type 2 infections in Portugal. J Gen Virol. 2003;84:1287–99.PubMedCrossRefGoogle Scholar
  17. Popper SJ, Sarr AD, Travers KU, Guèye-Ndiaye A, Mboup S, Essex ME, Kanki PJ. Lower human immunodeficiency virus (HIV) type 2 viral load reflects the difference in pathogenicity of HIV-1 and HIV-2. J Infect Dis. 1999;180(4):1116–21.PubMedCrossRefGoogle Scholar
  18. Schim van der Loeff M, Larke N, Kaye S, Berry N, Ariyoshi K, Alabi A, Van Tienen C, Leglidowicz A, Njie RS, da Silva Z, Jaye A, Vincent T, Rowland Jones S, Aaby P, Jaffar S, Whittle H. Undetectable plasma viral load predicts normal survival in HIV-2-infected people in a West African village. Retrovirology. 2010;19(7):46.CrossRefGoogle Scholar
  19. Sharp PM, Hahn BH. Origins of HIV and the AIDS pandemic. Cold Spring Harb Perspect Med. 2011;1(1):a006841.PubMedCrossRefPubMedCentralGoogle Scholar
  20. Tedder RS, O’Connor T, Hughes A, N’jie H, Corrah T, Whittle H. Envelope cross-reactivity in Western blot for HIV-1 and HIV-2 may not indicate dual infection. Lancet. 1988;2(8617):927–30.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Division of VirologyNational Institute for Biological Standards and ControlSouth MimmsUK