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Methods (In Vitro and In Vivo): Nephelometry and Turbidimetry

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Encyclopedia of Medical Immunology

Synonyms

Immune complex detection; Light scatter

Definition

Nephelometry and turbidimetry are analytical methods that involve instruments that detect the quantity of suspended particulate in a liquid or gas by measuring the magnitude of light beam scatter at different angles.

Introduction

Quantification of the concentration of analytes such as immunoglobulins (IgG, IgA, and IgM) and other proteins (amyloid) in human serum is commonly performed in the clinical immunology laboratory nephelometry or turbidimetry (Karamehic et al. 2012; Wang et al. 2012). Nephelometry and turbidimetry are analytical techniques that are based on the principle of the scattering of light by a solution containing dispersed particulate matter. When light is directed through a transparent medium containing solid particles, part of the light is scattered in all directions which gives a turbid appearance to the particulate mixture. The amount of decrease of the incident light related to scattering by particles in...

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References

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Author information

Authors and Affiliations

  1. Department of Medicine and Pathology, Johns Hopkins University School of Medicine; Johns Hopkins Dermatology, Allergy and Clinical Immunology Reference Laboratory; Johns Hopkins Asthma and Allergy Center, Room 1A20, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins, Baltimore, Maryland, 21224, USA

    Robert G. Hamilton

Authors
  1. Robert G. Hamilton
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Corresponding author

Correspondence to Robert G. Hamilton .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia

    Ian R. Mackay

  2. Center for Autoimmune Disease Research, Department of Molecular Microbiology and Immunology, Department of Pathology, Johns Hopkins University, Baltimore, MD, USA

    Noel R. Rose

  3. Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, James A. Haley Veterans' Hospital, Tampa, FL, USA

    Dennis K. Ledford

  4. Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, James A. Haley Veterans' Hospital, Tampa, FL, USA

    Richard F. Lockey

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Hamilton, R.G. (2014). Methods (In Vitro and In Vivo): Nephelometry and Turbidimetry. In: Mackay, I.R., Rose, N.R., Ledford, D.K., Lockey, R.F. (eds) Encyclopedia of Medical Immunology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-9194-1_301

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  • DOI: https://doi.org/10.1007/978-1-4614-9194-1_301

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-9193-4

  • Online ISBN: 978-1-4614-9194-1

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