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Encyclopedia of Malaria pp 1–15Cite as

Translation Initiation and Termination in the Human Malaria Parasite Plasmodium falciparum

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Synonyms

Protein synthesis; Start; Stop

Definition

The cell synthesizes several important bio polymers, among others: DNA using replication machinery, RNA using transcription machinery, and proteins using translation machinery. These synthesis processes have been divided into logical stages – initiation, elongation, and termination – with the building blocks being different for each polymer. Protein synthesis or translation, the focus of this article, is the process by which amino acid precursors are joined by peptide linkages to form proteins. The translation machinery consists of ribosomal proteins and ribosomal RNAs that act in conjunction with associated proteins at each step of translation: initiation factors that signal when and where to start translation, elongation factors that coordinate the addition of amino acids to the growing polypeptide chain, and termination factors that recognize stop codons.

While all these stages are highly regulated, the commitment to starting the...

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References

  • Aitken CE, Lorsch JR. A mechanistic overview of translation initiation in eukaryotes. Nat Struct Mol Biol. 2012;19:568–76.

    Article  CAS  PubMed  Google Scholar 

  • Amulic B, Salanti A, Lavstsen T, Nielsen MA, Deitsch KW. An upstream open reading frame controls translation of var2csa, a gene implicated in placental malaria. PLoS Pathog. 2009;5:e1000256.

    Article  PubMed Central  PubMed  Google Scholar 

  • Aravind L, Iyer LM, Wellems TE, Miller LH. Plasmodium biology: genomic gleanings. Cell. 2003;115:771–85.

    Article  CAS  PubMed  Google Scholar 

  • Aurrecoechea C, Brestelli J, Brunk BP, Dommer J, Fischer S, Gajria B, Gao X, Gingle A, Grant G, Harb OS, et al. PlasmoDB: a functional genomic database for malaria parasites. Nucleic Acids Res. 2009;37:D539–43.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Babbitt SE, Altenhofen L, Cobbold SA, Istvan ES, Fennell C, Doerig C, Llinas M, Goldberg DE. Plasmodium falciparum responds to amino acid starvation by entering into a hibernatory state. Proc Natl Acad Sci U S A. 2012;109:E3278–87.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Bawankar P, Shaw PJ, Sardana R, Babar PH, Patankar S. 5′ and 3′ end modifications of spliceosomal RNAs in Plasmodium falciparum. Mol Biol Rep. 2010;37:2125–33.

    Article  CAS  PubMed  Google Scholar 

  • Bender A, van Dooren GG, Ralph SA, McFadden GI, Schneider G. Properties and prediction of mitochondrial transit peptides from Plasmodium falciparum. Mol Biochem Parasitol. 2003;132:59–66.

    Article  CAS  PubMed  Google Scholar 

  • Chaubey S, Kumar A, Singh D, Habib S. The apicoplast of Plasmodium falciparum is translationally active. Mol Microbiol. 2005;56:81–9.

    Article  CAS  PubMed  Google Scholar 

  • Chaudhari R, Narayan A, Patankar S. A novel trafficking pathway in Plasmodium falciparum for the organellar localization of glutathione peroxidase-like thioredoxin peroxidase. FEBS J. 2012;279:3872–88.

    Article  CAS  PubMed  Google Scholar 

  • Cui L, Fan Q, Li J. The malaria parasite Plasmodium falciparum encodes members of the Puf RNA-binding protein family with conserved RNA binding activity. Nucleic Acids Res. 2002;30:4607–17.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Eliana C, Javier E, Moises W. Plasmodium falciparum spliceosomal RNAs: 3′ and 5′ end processing. Acta Trop. 2011;117:105–8.

    Article  CAS  PubMed  Google Scholar 

  • Evans L, Gowers D, Firman K, Youell J. Enhanced purification and characterization of the PfeIF4A (PfH45) helicase from Plasmodium falciparum using a codon-optimised clone. Protein Expr Purif. 2012;85:1–8.

    Article  CAS  PubMed  Google Scholar 

  • Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, Carlton JM, Pain A, Nelson KE, Bowman S, et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature. 2002;419:498–511.

    Article  CAS  PubMed  Google Scholar 

  • Ghersa P, Shrivastava IK, Perrin LH, Dobeli H, Becherer DJ, Matile H, Meier B, Certa U. Initiation of translation at a UAG stop codon in the aldolase gene of Plasmodium falciparum. EMBO J. 1990;9:1645–9.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Gomes-Santos CS, Braks J, Prudencio M, Carret C, Gomes AR, Pain A, Feltwell T, Khan S, Waters A, Janse C, et al. Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio. PLoS Pathog. 2011;7:e1002046.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ho CK, Shuman S. A yeast-like mRNA capping apparatus in Plasmodium falciparum. Proc Natl Acad Sci U S A. 2001;98:3050–5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jackson RJ, Hellen CU, Pestova TV. The mechanism of eukaryotic translation initiation and principles of its regulation. Nature reviews. Mol Cell Biol. 2010;11:113–27.

    CAS  Google Scholar 

  • Jackson KE, Habib S, Frugier M, Hoen R, Khan S, Pham JS, Ribas de Pouplana L, Royo M, Santos MA, Sharma A, et al. Protein translation in Plasmodium parasites. Trends Parasitol. 2011;27:467–76.

    Article  CAS  PubMed  Google Scholar 

  • Jackson KE, Pham JS, Kwek M, De Silva NS, Allen SM, Goodman CD, McFadden GI, de Pouplana LR, Ralph SA. Dual targeting of aminoacyl-tRNA synthetases to the apicoplast and cytosol in Plasmodium falciparum. Int J Parasitol. 2012;42:177–86.

    Article  CAS  PubMed  Google Scholar 

  • Kaiser A. Translational control of eIF5A in various diseases. Amino Acids. 2012;42:679–84.

    Article  CAS  PubMed  Google Scholar 

  • Kaiser A, Hammels I, Gottwald A, Nassar M, Zaghloul MS, Motaal BA, Hauber J, Hoerauf A. Modification of eukaryotic initiation factor 5A from Plasmodium vivax by a truncated deoxyhypusine synthase from Plasmodium falciparum: an enzyme with dual enzymatic properties. Bioorg Med Chem. 2007;15:6200–7.

    Article  CAS  PubMed  Google Scholar 

  • Kamali AN, Marin-Garcia P, Azcarate IG, Diez A, Puyet A, Bautista JM. Plasmodium yoelii blood-stage antigens newly identified by immunoaffinity using purified IgG antibodies from malaria-resistant mice. Immunobiology. 2012;217:823–30.

    Article  CAS  PubMed  Google Scholar 

  • Kehr S, Sturm N, Rahlfs S, Przyborski JM, Becker K. Compartmentation of redox metabolism in malaria parasites. PLoS Pathog. 2010;6:e1001242.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Knapp B, Gunther K, Lingelbach K. In vitro translation of Plasmodium falciparum aldolase is not initiated at an unusual site. EMBO J. 1991;10:3095–7.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Kochetov AV, Sarai A, Rogozin IB, Shumny VK, Kolchanov NA. The role of alternative translation start sites in the generation of human protein diversity. Mol Genet Genomics MGG. 2005;273:491–6.

    Article  CAS  Google Scholar 

  • Mair GR, Lasonder E, Garver LS, Franke-Fayard BM, Carret CK, Wiegant JC, Dirks RW, Dimopoulos G, Janse CJ, Waters AP. Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote development. PLoS Pathog. 2010;6:e1000767.

    Article  PubMed Central  PubMed  Google Scholar 

  • Mohrle JJ, Zhao Y, Wernli B, Franklin RM, Kappes B. Molecular cloning, characterization and localization of PfPK4, an eIF-2alpha kinase-related enzyme from the malarial parasite Plasmodium falciparum. Biochem J. 1997;328(Pt 2):677–87.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Molitor IM, Knobel S, Dang C, Spielmann T, Allera A, Konig GM. Translation initiation factor eIF-5A from Plasmodium falciparum. Mol Biochem Parasitol. 2004;137:65–74.

    Article  CAS  PubMed  Google Scholar 

  • Muller K, Matuschewski K, Silvie O. The Puf-family RNA-binding protein Puf2 controls sporozoite conversion to liver stages in the malaria parasite. PLoS One. 2011;6:e19860.

    Article  PubMed Central  PubMed  Google Scholar 

  • Nguyen TV, Fujioka H, Kang AS, Rogers WO, Fidock DA, James AA. Stage-dependent localization of a novel gene product of the malaria parasite, Plasmodium falciparum. J Biol Chem. 2001;276:26724–31.

    Article  CAS  PubMed  Google Scholar 

  • Patakottu BR, Mamidipally C, Patankar S, Noronha S. In silico analysis of translation initiation sites from P. falciparum. Online J Bioinform. 2009;10:259–79.

    Google Scholar 

  • Patakottu BR, Singh PK, Malhotra P, Chauhan VS, Patankar S. In vivo analysis of translation initiation sites in Plasmodium falciparum. Mol Biol Rep. 2012;39:2225–32.

    Article  CAS  PubMed  Google Scholar 

  • Pino P, Aeby E, Foth BJ, Sheiner L, Soldati T, Schneider A, Soldati-Favre D. Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA Met formylation in Apicomplexa. Mol Microbiol. 2010;76:706–18.

    Article  CAS  PubMed  Google Scholar 

  • Proud CG. eIF2 and the control of cell physiology. Semin Cell Dev Biol. 2005;16:3–12.

    Article  CAS  PubMed  Google Scholar 

  • Saul A, Battistutta D. Analysis of the sequences flanking the translational start sites of Plasmodium falciparum. Mol Biochem Parasitol. 1990;42:55–62.

    Article  CAS  PubMed  Google Scholar 

  • Scheper GC, van der Knaap MS, Proud CG. Translation matters: protein synthesis defects in inherited disease. Nat Rev Genet. 2007;8:711–23.

    Article  CAS  PubMed  Google Scholar 

  • Shaw PJ, Ponmee N, Karoonuthaisiri N, Kamchonwongpaisan S, Yuthavong Y. Characterization of human malaria parasite Plasmodium falciparum eIF4E homologue and mRNA 5′ cap status. Mol Biochem Parasitol. 2007;155:146–55.

    Article  CAS  PubMed  Google Scholar 

  • Shuman S. Capping enzyme in eukaryotic mRNA synthesis. Prog Nucleic Acid Res Mol Biol. 1995;50:101–29.

    Article  CAS  PubMed  Google Scholar 

  • Struck NS, Herrmann S, Langer C, Krueger A, Foth BJ, Engelberg K, Cabrera AL, Haase S, Treeck M, Marti M, et al. Plasmodium falciparum possesses two GRASP proteins that are differentially targeted to the Golgi complex via a higher- and lower-eukaryote-like mechanism. J Cell Sci. 2008;121:2123–9.

    Article  CAS  PubMed  Google Scholar 

  • Tuteja R. Identification and bioinformatics characterization of translation initiation complex eIF4F components and poly(A)-binding protein from Plasmodium falciparum. Commun Integr Biol. 2009;2:245–60.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tuteja R, Pradhan A. Isolation and functional characterization of eIF4F components and poly(A)-binding protein from Plasmodium falciparum. Parasitol Int. 2009;58:481–5.

    Article  CAS  PubMed  Google Scholar 

  • Tuteja R, Pradhan A. PfeIF4E and PfeIF4A colocalize and their double-stranded RNA inhibits Plasmodium falciparum proliferation. Commun Integr Biol. 2010;3:611–3.

    Article  PubMed Central  PubMed  Google Scholar 

  • Watanabe J, Suzuki Y, Sasaki M, Sugano S. Full-malaria 2004: an enlarged database for comparative studies of full-length cDNAs of malaria parasites, Plasmodium species. Nucleic Acids Res. 2004;32:D334–8.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhang M, Fennell C, Ranford-Cartwright L, Sakthivel R, Gueirard P, Meister S, Caspi A, Doerig C, Nussenzweig RS, Tuteja R, et al. The Plasmodium eukaryotic initiation factor-2alpha kinase IK2 controls the latency of sporozoites in the mosquito salivary glands. J Exp Med. 2010;207:1465–74.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhang M, Mishra S, Sakthivel R, Rojas M, Ranjan R, Sullivan Jr WJ, Fontoura BM, Menard R, Dever TE, Nussenzweig V. PK4, a eukaryotic initiation factor 2alpha(eIF2alpha) kinase, is essential for the development of the erythrocytic cycle of Plasmodium. Proc Natl Acad Sci U S A. 2012;109:3956–61.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zuegge J, Ralph S, Schmuker M, McFadden GI, Schneider G. Deciphering apicoplast targeting signals–feature extraction from nuclear-encoded precursors of Plasmodium falciparum apicoplast proteins. Gene. 2001;280:19–26.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay (IITB), Mumbai, India

    Swati Patankar, Aishwarya Narayan, Rahul Chaudhari & Rimi Chakrabarti

  2. Department of Microbiology, Monash University, Melbourne, Australia

    Rimi Chakrabarti

  3. IITB-Monash Research Academy, IIT Bombay, Mumbai, India

    Rimi Chakrabarti

Authors
  1. Swati Patankar
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  2. Aishwarya Narayan
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  3. Rahul Chaudhari
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  4. Rimi Chakrabarti
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Corresponding author

Correspondence to Swati Patankar .

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Editors and Affiliations

  1. Heswall, United Kingdom

    Marcel Hommel

  2. Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany

    Peter G. Kremsner

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Patankar, S., Narayan, A., Chaudhari, R., Chakrabarti, R. (2013). Translation Initiation and Termination in the Human Malaria Parasite Plasmodium falciparum . In: Hommel, M., Kremsner, P. (eds) Encyclopedia of Malaria. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8757-9_55-1

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  • DOI: https://doi.org/10.1007/978-1-4614-8757-9_55-1

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  • Online ISBN: 978-1-4614-8757-9

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