Synonyms
Erythrocyte, or Red blood cell; Fluorophore, or Fluorochrome or Chromophore; Giemsa blood smear, or Malaria smear or Blood film
Definition
Fluorescent microscopy for malaria diagnosis is the use of a microscope fitted with a source of ultraviolet (UV) radiation to aid in the examination of fluorescent blood specimens to detect malaria parasites. It may involve fluorescent labelling, a process of covalently attaching a fluorophore to a target molecule generally done by using a reactive derivative of the fluorophore that selectively binds to a functional group (contained in the target molecule). The molecules to be labeled are antibodies, proteins, amino acids, and peptides, which are then used as specific probes for detection of a particular target. [A fluorophore is a fluorescent chemical compound that can reemit light upon light excitation.]
Introduction
Giemsa microscopy and other Romanowsky staining methods, despite being the gold standard for malaria diagnosis, have...
References
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Wongsrichanalai, C., Kawamoto, F. (2014). Fluorescent Microscopy and Fluorescent Labelling for Malaria Diagnosis. In: Hommel, M., Kremsner, P. (eds) Encyclopedia of Malaria. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8757-9_119-1
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DOI: https://doi.org/10.1007/978-1-4614-8757-9_119-1
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