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Electron Tomography of Plant Cells

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Cell Biology

Abstract

Electron tomography (ET) is an emerging imaging approach to analyze the three-dimensional architecture of cells, organelles, and molecular complexes. Electron tomograms are calculated from multiple images of a specimen taken at different tilt angles. Both plastic-embedded and frozen-hydrated specimens can be imaged with this technique. This chapter deals with the general principle of electron tomography of biological samples and discusses the contributions of ET studies to advancing our knowledge on plant cell biology.

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Acknowledgments

Work on the electron tomographic analysis of plant cells in the Otegui laboratory is supported by grants MCB1157824 and IOS1339325 from the National Science Foundation.

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Authors and Affiliations

  1. Departments of Botany and Genetics, University of Wisconsin, B119 Birge Hall, 430 Lincoln Drive, Madison, WI, 53706-1381, USA

    Prof. Marisa S. Otegui

Authors
  1. Prof. Marisa S. Otegui
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Correspondence to Marisa S. Otegui .

Editor information

Editors and Affiliations

  1. Department of Biology, Pennsylvania State University, University Park, Pennsylvania, USA

    Sarah Assmann

  2. University of California Sect. Plant Biology, Davis, California, USA

    Bo Liu

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Otegui, M.S. (2014). Electron Tomography of Plant Cells. In: Assmann, S., Liu, B. (eds) Cell Biology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7881-2_12-1

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  • DOI: https://doi.org/10.1007/978-1-4614-7881-2_12-1

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  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-7881-2

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