Voltage-Sensitive Dye Imaging, Intrinsic Optical Signals
Voltage-sensitive dye imaging is an experimental technique to measure neuronal network activity from in vivo brains or live brain slices. In this technique, exogenous chemical agents are employed to continuously and rapidly transduce changes in cell membrane potential into changes in fluorescence emission.
Intrinsic optical imaging uses light at various wavelengths to probe activity-induced changes in the intrinsic optical properties of the brain tissues. The most common changes measured by intrinsic optical imaging are hemoglobin oxygenation and concentration because hemoglobin is a major chromophore in brain tissues within the visible wavelength region.
Voltage-sensitive dye imaging (VSDI) and intrinsic optical imaging (IOI) are both spatiotemporal neuroimaging methods. However, measurements from VSDI are direct reflections...
KeywordsDura Styryl Oxonol
- Berwick J, Johnston D, Jones M, Martindale J, Martin C, Kennerley AJ, Redgrave P, Mayhew JEW (2008) Fine detail of neurovascular coupling revealed by spatiotemporal analysis of the hemodynamic response to single whisker stimulation in rat barrel cortex. J Neurophysiol 99:787–798PubMedCrossRefPubMedCentralGoogle Scholar
- Frostig RD, Lieke EE, Ts’o DY, Grinvald A (1990) Cortical functional architecture and local coupling between neuronal activity and the microcirculation revealed by in vivo high-resolution optical imaging of intrinsic signals. Proc Natl Acad Sci U S A 87:6082–6086PubMedCrossRefPubMedCentralGoogle Scholar