Encyclopedia of Computational Neuroscience

Living Edition
| Editors: Dieter Jaeger, Ranu Jung

Voltage-Sensitive Dye Imaging, Intrinsic Optical Signals

  • Ian M. Devonshire
  • Ying Zheng
  • Jason Berwick
Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-7320-6_541-1



Voltage-sensitive dye imaging is an experimental technique to measure neuronal network activity from in vivo brains or live brain slices. In this technique, exogenous chemical agents are employed to continuously and rapidly transduce changes in cell membrane potential into changes in fluorescence emission.

Intrinsic optical imaging uses light at various wavelengths to probe activity-induced changes in the intrinsic optical properties of the brain tissues. The most common changes measured by intrinsic optical imaging are hemoglobin oxygenation and concentration because hemoglobin is a major chromophore in brain tissues within the visible wavelength region.

Detailed Description

Voltage-sensitive dye imaging (VSDI) and intrinsic optical imaging (IOI) are both spatiotemporal neuroimaging methods. However, measurements from VSDI are direct reflections...


Brain Slice Ocular Dominance Cell Membrane Potential Hemoglobin Oxygenation Neurovascular Coupling 
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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.School of Life SciencesQueen’s Medical CentreNottinghamUK
  2. 2.School of Systems EngineeringThe University of ReadingReadingUK
  3. 3.Department of PsychologyThe University of SheffieldSheffieldUK