Living reference work entry
The Process of Translation Initiation
Translation initiation in eukaryotic cells is defined as the process by which a 40S ribosomal subunit containing bound initiator Met-tRNAi (methionine is the first N-terminal amino acid of all synthesized protein chains) interacts with an mRNA and selects the start AUG codon to set the reading frame of protein synthesis. The process involves the formation of multiple noncovalent intermediate biochemical complexes in a series of distinct partial steps and requires the essential participation of a dozen protein factors, collectively called the eukaryotic translation Initiation Factors (eIFs). A schematic representation of the canonical pathway of translation initiation from a translationally competent eukaryotic capped-mRNA is depicted in Fig. 1.
- Bandyopadhyay A, Lakshmanan V, Matsumoto T, Chang EC, Maitra U. Moe1 and spInt6, the fission yeast homologs of mammalian translation initiation factor 3 subunits p66 (eIF3d) and p48 (eIF3e), respectively are required for stable association of eIF3 subunits. J Biol Chem. 2002;277:2360–7.CrossRefGoogle Scholar
- Choudhuri A, Evans T, Maitra U. An approach to reveal novel translational regulatory mechanisms in vertebrates: probing the function of eIF3 non-core subunits. In: 9th International Zebrafish Meeting on Development and Genetics, Madison. 2010.Google Scholar
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