Encyclopedia of Signaling Molecules

Living Edition
| Editors: Sangdun Choi

DREAM (Downstream Regulatory Element Antagonist Modulator)

  • Stephen Pastore
  • Hai-Ying Mary Cheng
Living reference work entry
DOI: https://doi.org/10.1007/978-1-4614-6438-9_101763-1


Historical Background

In 1998, Carrión et al. discovered a 110 kDa protein complex present in nuclear extracts of human neuroblastoma cells that could bind to a small region of DNA (+36 to +44 relative to the transcription start site) within the 5′ untranslated region (5′-UTR) of the human prodynorphin gene. Occupancy of this DNA element, termed the downstream regulatory element (DRE), by the protein complex caused a significant attenuation in prodynorphin gene transcription (Carrión et al. 1998). Utilizing a double-stranded DRE oligonucleotide probe to screen a human caudate expression library, Carrión et al. (1999) subsequently cloned the gene encoding for the DRE-binding protein, which they named downstream regulatory element antagonist modulator (DREAM). Electrophoretic mobility shift assays (EMSAs) confirmed that DREAM can bind to DRE and that the...


Downstream Regulatory Element Antagonist Modulator Neuronal Calcium Sensor Prodynorphin Gene Downstream Regulatory Element Charge Amino Acid Side Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Springer Science+Business Media LLC 2016

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Toronto MississaugaMississaugaCanada
  2. 2.Department of Cell and Systems BiologyUniversity of TorontoTorontoCanada