Chfr (Checkpoint with FHA and RING domains) is a ubiquitously expressed E3 ubiquitin ligase that is involved in a mitotic cell cycle checkpoint. It was identified from an expressed sequence tag (EST) database search for proteins containing forkhead-associated (FHA) motifs (Scolnick and Halazonetis 2000). Protein BLAST comparison revealed that Chfr is conserved across species, from unicellular eukaryotic yeast cell, Schizosaccharomyces pombe and Saccharomyces cerevisiaeto mice. Chfr orthologues in yeast, Dma1 and its paralog, Dma2, have been shown to be involved in regulating various cell division cycle processes such as spindle assembly, spindle positioning, and cytokinesis. More significantly, Dma1 and Dma2 were also demonstrated to regulate mitotic exit in fission yeast and budding yeast via SIN (Septation Initiation Network) and MEN (Mitotic Exit Network) respectively (analogous to...
KeywordsUbiquitin Ligase Translationally Control Tumor Protein Ring Domain Ubiquitin Ligase Activity Mitotic Entry
YFM is funded by the Singapore Ministry of Education Tier 2 grant (R183-000-328-112).
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