Spectroscopy of Damaged DNA
UV, fluorescence, circular dichroism, NMR, and other spectroscopic approaches can be used to study the properties of modified DNA (usually at the oligonucleotide level).
Long pieces of DNA with modifications are generally not very amenable to spectroscopy in that the spectral properties of the unmodified DNA bases obscure those of the damaged lesion. Most sophisticated spectroscopic studies have been done with oligonucleotides ≤18 bases in length and include ultraviolet (UV), fluorescence, circular dichroism (CD), and NMR studies. The synthesis of modified oligonucleotides is discussed under “Synthesis of Modified Oligonucleotides.”
KeywordsHPLC Titration Hydrocarbon Adduct Pyrene
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