Definition
The frequency of a particular type of damage is the number of molecules of the damage (adduct) occurring in a defined amount of DNA. This is a measure of the damage and is highly correlated with the resulting genotoxicity.
Discussion
As mentioned earlier, it is impossible to avoid all DNA damage. Even DNA samples from healthy individuals show significant levels of oxidized bases and other damage products. Many measurements have been done on levels of DNA damage under various settings with DNA from both humans and experimental animals. The question is often raised as to what level of DNA damage is a problem, particularly in regulatory settings. A precise answer cannot be given, in that the biological outcome is dependent not only on the frequency of DNA damage but also on (i) the chemical nature of the lesion, (ii) the site of the DNA base to which it is attached, (iii) the gene and position at which it is found, (iv) its rate of repair, and (v) phenomena such as the DNA...
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References
Billson HA, Harrison KL, Lees NP et al (2009) Dietary variables associated with DNA N7-methylguanine levels and O6-alkylguanine DNA-alkyltransferase activity in human colorectal mucosa. Carcinogenesis 30:615–620
Chastain PD 2nd, Nakamura J, Rao S et al (2010) Abasic sites preferentially form at regions undergoing DNA replication. FASEB J 24:3674–3680
Kim D-H, Guengerich FP (1990) Formation of the DNA adduct S-[2-(N7-guanyl)ethyl]glutathione from ethylene dibromide: effects of modulation of glutathione and glutathione S-transferase levels and the lack of a role for sulfation. Carcinogenesis 11:419–424
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Guengerich, F.P. (2014). Frequency of DNA Damage. In: Bell, E. (eds) Molecular Life Sciences. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6436-5_319-1
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DOI: https://doi.org/10.1007/978-1-4614-6436-5_319-1
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Publisher Name: Springer, New York, NY
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