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Control of Initiation in E. coli

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Molecular Life Sciences

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Synopsis

Studies have revealed several independent molecular mechanisms that regulate the frequency of replication initiation in E. coli (Skarstad and Katayama 2013; Nielsen and Lobner-Olesen 2008). By comparison with the biochemistry of DNA replication in eukaryotic cells, evidence suggests that all domains of life utilize comparable strategies to regulate the frequency of initiation. In E. coli, one pathway relies on the sequestration of the replication origin to occlude DnaA and other proteins from assembling at this site at an inappropriate time in the cell cycle. Sequestration occurs during the time interval that immediately follows initiation. Other biochemical pathways act by either modulating the availability of DnaA or its activity.

Introduction

DNA replication and the cell cycle.DNA replication in free-living organisms occurs only once and at a specific time in the cell cycle. This process can be formally divided into the stages of initiation of DNA replication, elongation...

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Acknowledgements

I thank the members of my lab for their support while I was writing. This work is supported by Grant GM090063 from the National Institutes of Health and by the Michigan Agricultural Experiment Station.

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  1. Department of Biochemistry & Molecular Biology, Michigan State University, 322 Biochemistry Building, 48823, East Lansing, MI, USA

    Jon M Kaguni

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  1. Dept of Chemistry, University of Richmond, Richmond, Virginia, USA

    Ellis Bell

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Kaguni, J.M. (2014). Control of Initiation in E. coli . In: Bell, E. (eds) Molecular Life Sciences. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6436-5_144-1

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