Definition
Subtractive hybridization magnetic bead capture (SHBMC) is a sequence-based metagenomic technique for the recovery of full-length ORFs from heterogeneous metagenomic DNA samples.
Introduction
It is widely acknowledged that the vast majority (~99 %) of microorganisms present in the environment are resistant to culture using classical microbiological methods. Approximately half of the total estimated bacterial phyla (61) are still to be cultured (Vartoukian et al. 2010). However, environmental microbial communities constitute a valuable resource for biotechnology and are a valid target for identification of novel genes and/or biological compounds such as biocatalysts or secondary metabolites (Sharma et al. 2005). In order to bypass the limitations of microbial culturing and to discover new microbial genes and functions, two approaches have been implemented, either culture-based, through the development of innovative...
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Cowan, D., Ronca, S., Ramond, JB. (2013). Subtractive Hybridization Magnetic Bead Capture: Molecular Technique for Recovery of Full-Length ORFs from Metagenomes. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_628-2
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DOI: https://doi.org/10.1007/978-1-4614-6418-1_628-2
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