MetaTISA: Metagenomic Gene Start Prediction with
Gene start: the start position from which a genomic sequence can be translated into protein.
Knowledge of exact information of gene start plays an important role in identification of native purified proteins from the high-throughput proteomics (Poole et al. 2005). In addition, a correct prediction of gene start facilitates the identification of cis-regulatory signals related to translation initiation (Hu et al. 2008c) and thus facilitates the understanding of the diversity and evolution scenario of translation initiation mechanisms (Zheng et al. 2011). However, gene start annotation in widely used public databases such as GenBank and RefSeq is not of high quality in general (Nielsen and Krogh 2005). In particular, the longest open reading frame is frequently used to annotate a protein-coding gene (Besemer et al. 2001), which results in a systematical low quality in gene start...
KeywordsGene Start Phylogenetic Origin Metagenomic Sample Initial Annotation Longe Open Reading Frame
- Hu G, Liu Y, et al. New solutions of translation initiation site prediction for prokaryotic genomes. Prog Biochem Biophys. 2008a;35(11):1254–62.Google Scholar