Definition
Soil metagenomes are a major source of various enzymes and biomolecules. Lipases are among one of the most investigated class of enzymes in metagenomics. A large number of structurally and functionally diverse lipases from metagenomes have contributed to the discovery of a new lipase families that do not fit into any of the existing eight families.
Introduction
Lipases are the most versatile enzymes found in animals, plants, and microorganisms. They are considered as one of the most important biocatalysts in biotechnological application. Lipases utilize a wide range of substrates; show chemo-, regio- and enantioselectivity; and degrade acyl p-nitrophenyl esters, tweens, and phospholipids. In addition to their natural function of hydrolyzing carboxylic ester bonds, lipases can also catalyze, alcoholysis, acidolysis, esterification, or transesterification reactions under different conditions. These variety of...
This is a preview of subscription content, log in via an institution.
References
Arpigny JL, Jaeger KE. Bacterial lipolytic enzymes: classification and properties. Biochem J. 1999;343:177–83.
Berlemont R, Pipers D, Delsaute M, et al. Exploring the Antarctic soil metagenome as a source of novel cold-adapted enzymes and genetic mobile elements. Rev Argent de Microbiol. 2011;43:94–103.
Berlemont R, Jacquin O, Delsaute M, et al. Novel cold-adapted esterase MHlip from an Antarctic soil metagenome. Biology. 2013;2:177–88.
Chow J, Kovacic F, Antonia DY, et al. The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases. PLoS ONE. 2012;7(10):1–16.
Cieslinski H, Bialkowska A, Tkaczuk K, et al. Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library. Pol J Microbiol. 2009;58(3):199–204.
Elend C, Steele HL, Schmeisser C, et al. Isolation and characterization of a metagenome-derived and cold-active lipase with high stereospecificity for (R)-ibuprofen esters. Biotechnol J. 2007;130:370–7.
Ewis HE, Abdelal AT, Lu CD. Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus. Gene. 2004;329:187–95.
Fan XJ, Liu XL, Wang K, et al. Highly soluble expression and molecular characterization of an organic solvent-stable and thermotolerant lipase originating from the metagenome. J Mol Catal B Enzym. 2011;72:319–26.
Faoro H, Glogauer A, Souza EM, et al. Identification of a new lipase family in the Brazilian Atlantic Forest soil metagenome. Environ Microbiol Rep. 2011;3(6):750–5.
Faoro H, Glogauer A, Couto GH, et al. Characterization of a new Acidobacteria-derived moderately thermostable lipase from a Brazilian Atlantic Forest soil metagenome. FEMS Microbiol Ecol. 2012;81:386–94.
Glogauer A, Martini VP, Faoro H, et al. Identification and characterization of a new true lipase isolated through metagenomic approach. Microb Cell Fact. 2011;10:54–68.
Handelsman J. Metagenomics: application of genomics to uncultured microorganisms. Microbiol Mol Biol Rev. 2004;68:669–85.
Handrick R, Reinhardt S, Focarete ML, et al. A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. J Biol Chem. 2001;276:36215–24.
Henne A, Schmitz RA, Bomeke M, et al. Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli. Appl Environ Microbiol. 2000;66:3113–6.
Kang CH, Oh KH, Lee MH, et al. A novel family VII esterase with industrial potential from compost metagenomic library. Microb Cell Fact. 2011;10:41–8.
Khan M, Kottur J. Expression and purification of organic solvent stable lipase from soil metagenomic library. World J Microbiol Biotechnol. 2012;28:2417–24.
Khan M, Kottur J, et al. Cloning and characterization of two functionally diverse lipases from soil metagenome. J Gen Appl Microbiol. 2013;59:21–31.
Kim EY, Oh KH, et al. Novel cold-adapted alkaline lipase from an intertidal flat metagenome and proposal for a new family of bacterial lipases. Appl Environ Microbiol. 2009;75:257–260.
Lee MH, Lee CH, et al. Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases. Appl Environ Microbiol. 2006;72(11):7406–7409.
Lee SW, Won K, et al. Screening for novel lipolytic enzymes from uncultured soil microorganisms. Appl Microbiol Biotechnol. 2004;65:720–6.
Liu P, Wang YF, Ewis HE, et al. Covalent reaction intermediate revealed in crystal structure of the Geobacillus stearothermophilus carboxylesterase Est30. J Mol Biol. 2004;342:551–61.
Rao L, Xue Y, Zhou C, Tao J, Li G, et al. A thermostable esterase from Thermoanaerobacter tengcongensis opening up a new family of bacterial lipolytic enzymes. Biochim Biophys Acta. 2011;1814:1695–702.
Rhee JK, Ahn DG, Kim YG, Oh JW. New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library. Appl Environ Microbiol. 2005;71:817–25.
Rondon MR, August PR, Bettermann AD, et al. Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms. Appl Environ Microbiol. 2000;66:2541–7.
Selvin J, Kennedy J, Lejon DPH, et al. Isolation identification and biochemical characterization of a novel halo-tolerant lipase from the metagenome of the marine sponge Haliclona simulans. Microb Cell Fact. 2012;11:72–85.
Sharma PK, Singh K, Singh R, et al. Characterization of a thermostable lipase showing loss of secondary structure at ambient temperature. Mol Biol Rep. 2012;39:2795–804.
Voget S, Leggewie C, Uesbeck A, et al. Prospecting for novel biocatalysts in a soil metagenome. Appl Environ Microbiol. 2003;69:6235–42.
Wei P, Bai L, et al. Characterization of two soil metagenome-derived lipases with high specificity for p-nitrophenyl palmitate. Arch Microbiol. 2009;191:233–40.
Yuhong, Zhang, Shi P, et al. Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture. J Microbiol Biotechnol. 2009;19(9):888–97.
Zheng J, Liu L, et al. Molecular Cloning and heterologous expression of a true lipase in Pichia pastoris isolated via a metagenomic approach. J Mol Microbiol Biotechnol. 2012;22:300–11.
Zuo K, Zhang L, et al. Isolation and functional expression of a novel lipase gene isolated directly from oil-contaminated soil. Acta Biochim Pol. 2010;57(3):305–11.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this entry
Cite this entry
Angelina, Khan, M. (2013). Functionally Diverse Lipase from Soil Metagenome. In: Nelson, K. (eds) Encyclopedia of Metagenomics. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6418-1_219-4
Download citation
DOI: https://doi.org/10.1007/978-1-4614-6418-1_219-4
Received:
Accepted:
Published:
Publisher Name: Springer, New York, NY
Online ISBN: 978-1-4614-6418-1
eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences