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Optical Coherence Microscopy

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Handbook of Coherent-Domain Optical Methods

Abstract

This chapter presents the practical embodiment of two types of optical coherence microscope (OCM) modality that differ by probing method. The development and creation of a compact OCM device for imaging internal structures of biological tissue at the cellular level is presented. Ultrahigh axial resolution of 3.4 μm and lateral resolution of 3.9 μm within tissue was attained by combining broadband radiations of two spectrally shifted SLDs and implementing the dynamic focus concept, which allows in-depth scanning of a coherence gate and beam waist synchronously. This OCM prototype is portable and easy to operate; creation of a remote optical probe was feasible due to use of polarization maintaining fiber. The chapter also discusses the results of a theoretical investigation of OCM axial and lateral resolution degradation caused by light scattering in biological tissue. We demonstrate the first OCM images of biological objects using examples of plant and human tissue ex vivo.

Another variant of OCM is based on a broadband digital holographic technique. The final section of the chapter concerns 2D or 3D optical coherence tomography (OCT) imaging of the internal structure of strongly scattering media with micrometer-scale resolution by processing 200 sets of 2D holographic complex reconstructions at interference reception of backscattered light obtained at different wavelengths separated by a fixed spectral interval in the wavelength region of tens of nanometers. This technique of internal structure visualization apparently has a number of advantages over the known time-domain and spectral OCT methods, including the absence of transverse scanning systems at 3D visualization, and transverse spatial resolution has no limitations inherent in the correlation and spectral OCT techniques.

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Acknowledgments

The authors thank Alexander Turkin and Pavel Morozov for assistance in creating optical elements, Irina Andronova for valuable scientific discussion, Nadezhda Krivatkina and Lidia Kozina for providing translation, and Marina Chernobrovtzeva for editing. This work was partly supported by the Russian Foundation for Basic Research under the grants #01-02-17721, #03-02-17253, #03-02-06420 and by the Civilian Research & Development Foundation under the grant RB2-2389-NN-02 and State Contract No. 02.740.11.0516 of March 15, 2010.

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Authors and Affiliations

  1. Institute of Applied Physics of the Russian Academy of Sciences, Nizhny Novgorod, 603950, Russian Federation

    Grigory V. Gelikonov, Valentin M. Gelikonov, Sergey U. Ksenofontov, Andrey N. Morosov, Alexey V. Myakov, Yury P. Potapov, Veronika V. Saposhnikova, Ekaterina A. Sergeeva, Dmitry V. Shabanov & Natalia M. Shakhova

  2. Medical Academy, Nizhny Novgorod, 603005, Russian Federation

    Elena V. Zagainova

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  1. Grigory V. Gelikonov
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  2. Valentin M. Gelikonov
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  3. Sergey U. Ksenofontov
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  4. Andrey N. Morosov
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  5. Alexey V. Myakov
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  6. Yury P. Potapov
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  7. Veronika V. Saposhnikova
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  8. Ekaterina A. Sergeeva
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  9. Dmitry V. Shabanov
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  11. Elena V. Zagainova
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Correspondence to Grigory V. Gelikonov .

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Editors and Affiliations

  1. , Chair of Optics and Biophotonics, Saratov State University, Astrakhanskaya str. 83, Saratov, 410012, Russia

    Valery V. Tuchin

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Gelikonov, G.V. et al. (2013). Optical Coherence Microscopy. In: Tuchin, V. (eds) Handbook of Coherent-Domain Optical Methods. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5176-1_27

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