Silver in Protein Detection Methods in Proteomics Research
Proteomics is defined as the large-scale analysis of proteins and relies, in its final experimental step, on the identification of peptides by mass spectrometry. However, biological samples are so complex that the separating power of the mass spectrometer alone cannot handle this complexity, so that extensive fractionation of the proteins and/or peptides must be carried out prior to the mass spectrometry analysis.
One of the classical setups for proteomic analysis is based on the high-resolution separation of proteins by two-dimensional gel electrophoresis, combining isoelectric focusing in the first dimension and SDS electrophoresis on polyacrylamide gels in the second dimension. In this setup, prior to spot cutting and analysis by mass spectrometry, the proteins are detected and quantitatively analyzed directly on the second dimension polyacrylamide gels by various methods that can use...
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