Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Silver in Protein Detection Methods in Proteomics Research

Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_571

Synonyms

Definition

Proteomics is defined as the large-scale analysis of proteins and relies, in its final experimental step, on the identification of peptides by mass spectrometry. However, biological samples are so complex that the separating power of the mass spectrometer alone cannot handle this complexity, so that extensive fractionation of the proteins and/or peptides must be carried out prior to the mass spectrometry analysis.

One of the classical setups for proteomic analysis is based on the high-resolution separation of proteins by two-dimensional gel electrophoresis, combining isoelectric focusing in the first dimension and SDS electrophoresis on polyacrylamide gels in the second dimension. In this setup, prior to spot cutting and analysis by mass spectrometry, the proteins are detected and quantitatively analyzed directly on the second dimension polyacrylamide gels by various methods that can use...

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References

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.CNRS, UMR 5249 Laboratory of Chemistry and Biology of MetalsGrenobleFrance
  2. 2.CEA, DSV, iRTSV/LCBM, Chemistry and Biology of MetalsGrenoble Cedex 9France
  3. 3.Université Joseph FourierGrenobleFrance