Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Calcium-Regulated Photoproteins

  • Eugene S. VysotskiEmail author
  • John Lee
Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_55



Ca2+-regulated photoprotein is a “precharged” bioluminescent protein from which light emission is triggered by addition of calcium ions. The bioluminescence reaction does not require addition of molecular oxygen or any other cofactors, only the photoprotein and the triggering ion are necessary.


Bioluminescence is widespread in the biosphere. Luminous organisms have been found among bacteria, fungi, protozoa, coelenterates, worms, mollusks, insects, and fish. Although these organisms occupy different places in the evolutionary ladder, the nature of their bioluminescence is always the same. In fact, bioluminescence is a chemiluminescent reaction whereby oxidation of a substrate, luciferin, is catalyzed by a specific enzyme, luciferase. Luciferins and luciferases of different organisms differ in structure; that is, the terms are generic and functional rather than structural and chemical. The many differences suggest that...

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  1. Blinks JR, Wier WG, Hess P, Prendergast FG (1982) Measurement of Ca2+ concentrations in living cells. Prog Biophys Mol Biol 40:1–114CrossRefPubMedGoogle Scholar
  2. Dupriez VJ, Maes K, Le Poul E, Burgeon E, Detheux M (2002) Aequorin-based functional assays for G-protein-coupled receptors, ion channels, and tyrosine kinase receptors. Receptors Channels 8:319–330CrossRefPubMedGoogle Scholar
  3. Hastings JW, Morin JG (1969) Calcium-triggered light emission in Renilla. A unitary biochemical scheme for coelenterate bioluminescence. Biochem Biophys Res Commun 37:493–498CrossRefPubMedGoogle Scholar
  4. Kawasaki H, Nakayama S, Kretsinger RH (1998) Classification and evolution of EF-hand proteins. Biometals 11:277–295CrossRefPubMedGoogle Scholar
  5. Markova SV, Vysotski ES, Blinks JR, Burakova LP, Wang BC, Lee J (2002) Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins. Biochemistry 41:2227–2236CrossRefPubMedGoogle Scholar
  6. Nelson MR, Chazin WJ (1998) Structures of EF-hand Ca2+-binding proteins: diversity in the organization, packing and response to Ca2+ binding. Biometals 11:297–318CrossRefPubMedGoogle Scholar
  7. Shimomura O (2006) Bioluminescence: chemical principles and methods. World Scientific, SingaporeCrossRefGoogle Scholar
  8. Strynadka NCJ, James MNG (1989) Crystal structures of the helix-loop-helix calcium-binding proteins. Annu Rev Biochem 58:951–998CrossRefPubMedGoogle Scholar
  9. Vysotski ES, Lee J (2004) Ca2+-regulated photoproteins: structural insight into the bioluminescence mechanism. Acc Chem Res 37:405–415CrossRefPubMedGoogle Scholar
  10. Vysotski ES, Lee J (2007) Bioluminescence mechanism of Ca2+-regulated photoproteins from three-dimensional structures. In: Viviani VR, Ohmiya Y (eds) Luciferases and fluorescent proteins: principles and advances in biotechnology and bioimaging. Transworld Research Network, KeralaGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Photobiology LaboratoryInstitute of Biophysics Russian Academy of Sciences, Siberian BranchKrasnoyarskRussia
  2. 2.Department of Biochemistry and Molecular BiologyUniversity of GeorgiaAthensUSA