Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov


  • ChulHee KangEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_38




Calsequestrin, a ~40 kD protein, binds Ca2+ with high capacity (40–50 mol Ca2+ mol−1 calsequestrin) but with moderate affinity (Kd = 1 mM) over a Ca2+ concentration range between 0.01 and 1 M and releases it with a high off-rate (106 s−1). A high concentration of calsequestrin (up to 100 mg mL−1) is present in the lumen of the junctional terminal cisternae of the sarcoplasmic reticulum (SR) (MacLennan and Chen 2009; Royer and Ríos 2009). In humans, there are two major isoforms; the skeletal muscle calsequestrin (Casq1) and the cardiac muscle calsequestrin (Casq2). They are encoded by casq1 and casq2 located on chromosomes 1q21 and 1p13.3-p11 and are the only isoforms in fast-twitch skeletal muscle and cardiac muscle, respectively. In slow-twitch skeletal muscle, Casq1 is the major isoform with Casq2 as minor. In general, the Ca2+-binding capacity of Casq2 is higher than that of Casq1. Isoforms of calsequestrin are also present in the endoplasmic...

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© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Washington State UniversityPullmanUSA