EF-hand: helix-loop-helix motif obeying a 29-residue consensus sequence with hydrophobic and Ca2+-ligating residues in defined positions, typically forming a pentagonal bipyramidal coordination sphere.
Hexa EF-hand (HEF) proteins: Ca2+-binding proteins containing six EF-hand motifs.
Domain: Independent folding unit.
Subdomain: Super-secondary motif which is part of a domain.
Deamidation: Spontaneous hydrolysis of a carboxamide to a carboxylic acid via a cyclic intermediate. Deamidation of asparagine typically leads to iso-aspartate and aspartate in a 2:1 molar ratio.
Chromophoric chelator: Metal-binding dye which changes its optical spectrum upon chelation.
Discovery and Prevalence
Calbindin D28k was originally purified from chicken intestine (Wasserman and Taylor 1996)...
- Permyakov EA, Kretsinger RH (2011) EF-hand proteins. In: Uversky VN (ed) Calcium binding proteins, Chapter 11, Section 11.5, Proteins with Six EF-Hands. Wiley, SingaporeGoogle Scholar