Encyclopedia of Metalloproteins

2013 Edition
| Editors: Robert H. Kretsinger, Vladimir N. Uversky, Eugene A. Permyakov

Calcium-Binding Protein Site Types

Reference work entry
DOI: https://doi.org/10.1007/978-1-4614-1533-6_35


Calcium:  Ca2+;  Ca(II); Chelate:  Bind;  Coordinate


Calcium: A soft gray alkaline earth metal. Ionic calcium (Ca2+) is essential for numerous cellular functions, as well as biomineralization.

Calcium-binding protein: Any protein or enzyme that requires the binding of a calcium ion to fulfill either a functional or structural role.

Binding site: Region in the protein where metal ions will bind as a result of electrostatic interactions with ligands of opposite charge.

Ligand: Ion, atom, or molecule that binds to a metal ion to form a coordination complex.

Tertiary structure: Three-dimensional shape of a protein, enzyme, or molecule.

Diverse Roles of Calcium-Binding Proteins

In the intracellular environment, calcium functions as a second messenger to facilitate a wide variety of functions related to muscle contraction, neurotransmitter release, and enzyme activation. Additionally, calcium and calcium-binding proteins (CaBPs) are involved in almost every aspect of the...

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  1. Forsen S, Linse S et al (1991) Ca2+ binding in proteins of the calmodulin superfamily: cooperativity, electrostatic contributions and molecular mechanisms. Ciba Found Symp 161:222–236PubMedGoogle Scholar
  2. Kirberger M, Wang X et al (2008) Statistical analysis of structural characteristics of protein Ca(2+)-binding sites. J Biol Inorg Chem 13(7):1169–1181CrossRefPubMedGoogle Scholar
  3. Kretsinger RH, Nockolds CE (1973) Carp muscle calcium-binding protein. II. Structure determination and general description. J Biol Chem 248(9):3313–3326PubMedGoogle Scholar
  4. Linse S, Helmersson A et al (1991) Calcium binding to calmodulin and its globular domains. J Biol Chem 266(13):8050–8054PubMedGoogle Scholar
  5. Mellanby J, Woolley VJ (1913) The ferments of the pancreas: Part II. The action of calcium salts in the generation of trypsin from trypsinogen. J Physiol 46(2):159–172PubMedGoogle Scholar
  6. Permyakov EA, Kretsinger RH (2010) Calcium binding proteins. Wiley, HobokenCrossRefGoogle Scholar
  7. Schreiber M, Salkoff L (1997) A novel calcium-sensing domain in the BK channel. Biophys J 73(3):1355–1363CrossRefPubMedGoogle Scholar
  8. Shin S, Feng M et al (2010) The tightly bound calcium of MauG Is required for tryptophan tryptophylquinone cofactor biosynthesis. Biochemistry 50:144–150CrossRefPubMedGoogle Scholar
  9. Stathopulos PB, Zheng L et al (2008) Structural and mechanistic insights into STIM1-mediated initiation of store-operated calcium entry. Cell 135(1):110–122CrossRefPubMedGoogle Scholar
  10. Ye Y, Lee HW et al (2005) Probing site-specific calmodulin calcium and lanthanide affinity by grafting. J Am Chem Soc 127(11):3743–3750CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of ChemistryGeorgia State UniversityAtlantaUSA
  2. 2.Natural Science CenterAtlantaUSA