Pathway Modeling, Metabolic
Metabolic pathways or network is composed of a number of enzyme-catalyzed reactions that utilize substrate (such as glucose, sucrose, acetic acid, etc.) to demonstrate a characteristic phenotype (such as growth or adaptation to stress) and in the process accumulate products (such as amino acids, ethanol, lactic acid, etc.). Characterization and quantification of all the reaction rates ( Metabolic Flux Analysis) of the metabolic network comes under metabolic pathway modeling. Approaches like metabolic flux analysis, 13C metabolic flux analysis, flux balance analysis, and metabolic pathway analysis are some of the approaches of metabolic pathway modeling, which assume pseudo-steady state condition for internal metabolites and are based on the principle of mass balance. Through such modeling techniques, it is possible to study metabolic network properties, robustness, and fragility in the network....
- Krömer J, Quek L-E, Nielsen L (2009) 13 C-fluxomics: a tool for measuring metabolic phenotypes. Aust Biochem 40(3):17–20Google Scholar
- Schuster S, Hilgetag C (1994) On elementary flux modes in biochemical reaction systems at steady state. J Biol Syst 2(2):165–182Google Scholar
- Stephanopoulos GN, Aristidou AA, Nielsen J (1998) Metabolic engineering: principles and methodologies, 1st edn. Academic, San DiegoGoogle Scholar