Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota

Perturbation

  • Rui-Sheng Wang
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_385

Synonyms

Definition

In systems biology, perturbation is an alteration of the function of a biological system by external or internal means such as environmental stimuli, drug inhibition, and gene knockdown. It is an approach often adopted in systems biology. In reverse engineering of gene regulatory networks, when a few genes in a cellular system are systematically perturbed, responses such as transcript levels from other parts of the system can be recorded to reconstruct a regulatory network diagram that more clearly depicts the relationships between genes. Such systematic perturbations in cells can also help to identify drug mode of action. In addition, genetic perturbations such as gene deletion, gene overexpression, and insertion mutation can be used to determine the function of a gene through experimental techniques.

Cross-References

References

  1. Gardner TS, di Bernardo D, Lorenz D, Collins JJ (2003) Inferring genetic networks and identifying compound mode of action via expression profiling. Science 301:102–105PubMedGoogle Scholar
  2. Ge H, Walhout AJM, Vidal M (2003) Integrating ‘omic’ information: a bridge between genomics and systems biology. Trends Genet 19:551–560PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of PhysicsPennsylvania State UniversityUniversity ParkUSA