Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota

Phosphodiester Bond Formation

  • Tetsuro Kokubo
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_1598



MRNA synthesis proceeds via one-by-one addition of appropriate (i.e., complementary to the template DNA strand) ribonucleotides (rNTPs) to the 3’-end. The phosphodiester bond (PO 43-) is formed by an S N2 reaction in which the hydroxyl group at the 3’-end of the mRNA attacks the α-phosphoryl group of the incoming rNTP (Fig.  1) (Watson et al. 2008). Pyrophosphate is released from rNTP upon phosphodiester bond formation.
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  1. Watson JD, Baker TA, Bell SP, Gann A, Levine M, Losick R (2008) Molecular biology of the gene, 6th edn. Cold Spring Harbor Laboratory Press, NY, 197Google Scholar

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© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Supramolecular BiologyGraduate School of Nanobioscience, Yokohama City UniversityYokohamaJapan