Proteomics, Quantification-Unbiased and Target Approach
One of the most important challenges in systems biology is the mass-spectrometry-based ( Mass Spectrometry, Proteomics, and Metabolomics) quantification of as many proteins as possible of a complex sample – called proteome quantification. There are basically two different ways to quantify a proteome: the relative and absolute quantification. The relative quantification allows for the comparative analysis of proteins throughout different samples/treatments. The relative quantification is a more unbiased approach which includes all detectable proteins of an analysis. The absolute quantification is a targeted approach that also enables the stoichiometrical elucidation of selected proteins within the same sample in terms of absolute concentration levels.
Proteome quantification aims at the assessment of relative or absolute abundances of the elements of a system’s total protein complement. Various...
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