Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota

Proteomics, Quantification-Unbiased and Target Approach

  • Stefanie Wienkoop
  • Christiana Staudinger
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_1151

Synonyms

Definition

One of the most important challenges in systems biology is the mass-spectrometry-based ( Mass Spectrometry, Proteomics, and Metabolomics) quantification of as many proteins as possible of a complex sample – called proteome quantification. There are basically two different ways to quantify a proteome: the relative and absolute quantification. The relative quantification allows for the comparative analysis of proteins throughout different samples/treatments. The relative quantification is a more unbiased approach which includes all detectable proteins of an analysis. The absolute quantification is a targeted approach that also enables the stoichiometrical elucidation of selected proteins within the same sample in terms of absolute concentration levels.

Characteristics

Proteome quantification aims at the assessment of relative or absolute abundances of the elements of a system’s total protein complement. Various...

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References

  1. Hoehenwarter W, Wienkoop S (2010) Spectral counting robust on high mass accuracy mass spectrometers. Rapid Commun Mass Spectrom 24:3609–3614PubMedGoogle Scholar
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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Molecular Systems BiologyUniversity of ViennaViennaAustria