Encyclopedia of Systems Biology

2013 Edition
| Editors: Werner Dubitzky, Olaf Wolkenhauer, Kwang-Hyun Cho, Hiroki Yokota


  • Lars Malmström
  • Andreas Quandt
  • Ela Pustulka-Hunt
Reference work entry
DOI: https://doi.org/10.1007/978-1-4419-9863-7_1003


Proteomics (Twyman 2004; Mishra 2010) is the study of a proteome, that is, the ensemble of proteins produced by an organism, a system or its subpart, and of the interactions between proteins within the system. The most common use of the term refers to the study of a collection of proteins that vary with time and correspond to internal or external cell processes and modifications. Such changes may be reflected in the presence or absence of a protein, its expression level, and in a variety of post-translational modifications, such as phosphorylation, ubiquitination, methylation, acetylation, glycosylation, oxidation, or nitrosylation.

Common proteomics methods include the use of antibodies that recognize some proteins or protein classes, gel electrophoresis in two dimensions, shotgun proteomics, western blot, immunohistochemical staining, enzyme linked immunosorbent assay (ELISA), and mass spectrometry (MS).



  1. Mishra NC (2010) Introduction to proteomics: principles and applications. Wiley, HobokenGoogle Scholar
  2. Twyman RM (2004) Principles of proteomics, Advanced text series. Taylor & Francis, New YorkGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Lars Malmström
    • 1
  • Andreas Quandt
    • 1
  • Ela Pustulka-Hunt
    • 2
  1. 1.Institute of Molecular Systems Biology IMSBETH ZurichZurichSwitzerland
  2. 2.GS SystemsX, ETH ZurichZurichSwitzerland