Gamborg Medium (B5)

Developed for plant tissue culture, it is suitable for growing callus and different plant organs. Composition mg/L: KNO₃ 2500, CaCl₂.2H₂O 150, MgSO₄.7H₂O 250, (NH₄)₂SO₄ 134, NaH₂PO₄.H₂O 150, KI 0.75, H₃BO₃ 3.0, MnSO₄.H₂O 10, ZnSO₄.7H₂O 2.0, Na₂MoO₄.2H2O 0.25, CuSO4.5H2O 0.025, CoCl2.6H2O 0.025, Ferric-EDTA 43, sucrose 2%, pH 5.5, inositol 100, nicotinic acid 1.0, pyridoxine.HCl 1.0, thiamine. HCl 10, kinetin 0.1, 2,4-D 0.1 - 1.0. The microelements, vitamins and hormones may be prepared in a stock solution and added before use. For kinetin other cytokinins may be substituted such as 6-benzylamino purine (BA) or isopentenyl adenine (or its nucleoside), for 2,4-D (dichloro-phenoxy acetic acid), naphthalene acetic acid (NAA) or indole acetic acid (IAA) may be substituted or a combination of the hormones may used in concentrations that is best suited for the plant and the purpose of the culture. For solid media use agar or gellan gum. Heat labile components are sterilized by filtering...