By homology it recognizes and then replaces a particular segment (gene) of the target. It has a pair of restriction enzyme recognition sites within the region of “non-essential” genes. Non-essential means that their removal and replacement do not impair packaging and propagation in E. coli by sequences of interest to the experimenter. vectors, stuffer
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(2008). Replacement Vector. In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6754-9_14402
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DOI: https://doi.org/10.1007/978-1-4020-6754-9_14402
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