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Arabidopsis Mutagen Assays

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In the mature embryo of plants two diploid cells represent the inflorescence. Therefore, if the seeds are exposed at such a stage to a mutagen, in the progeny of the emerging plants the segregation is about 7:1 for recessive mutations. This also indicates that one of the two apical cells became heterozygous for the new mutation induced. For mutagen assays it is sufficient to open up the immature fruits of the plants before the seed coat becomes opaque (about 10–14 days after fertilization) and albina or other color mutations in the cotyledons or embryo defects can be determined. Within a single fruit the segregation for recessives is 3:1 (see above). The fruits on the plants emerging from the treated seed already contain the F2generation. Generally, two opposite fruits next to each other are examined because the phyllotaxy index assures that these are sufficient for complete sampling. Such a test permits the identification of about 80% of the spectrum of visible mutations. Since the...

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Figure A113.

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(2008). Arabidopsis Mutagen Assays. In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6754-9_1088

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