Encyclopedia of Microfluidics and Nanofluidics

2008 Edition
| Editors: Dongqing Li

Amperometric Techniques

  • Thomas J. Roussel
  • Douglas J. Jackson
  • Richard P. Baldwin
  • Robert S. Keynton
Reference work entry
DOI: https://doi.org/10.1007/978-0-387-48998-8_26


Amperometric detection; Controlled-potential instrumentation; Electrochemical detection; Oxidation/reduction


Amperometry is one of a family of electrochemical methods in which the potential applied to a sensing electrode is controlled instrumentally and the current occurring as a consequence of oxidation/reduction at the electrode surface is recorded as the analytical signal. In its simplest form, the applied potential is stepped to and then held at a constant value; and resulting current is measured as a function of time. When amperometric detectionis used in conjunction with separation techniques such as capillary electrophoresis or liquid chromatography, the sensing (or “working”) electrode is placed at the end of the separation stream so as to permit detection of sample components as they emerge and pass over the electrode surface. The applied potential must be sufficient to cause facile oxidation or reduction of the target analytes; and, in general, the...

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Thomas J. Roussel
    • 1
    • 3
  • Douglas J. Jackson
    • 2
  • Richard P. Baldwin
    • 3
  • Robert S. Keynton
    • 1
  1. 1.Department of BioengineeringUniversity of LouisvilleLouisvilleUSA
  2. 2.Department of Electrical and Computer EngineeringUniversity of LouisvilleLouisvilleUSA
  3. 3.Department of ChemistryUniversity of LouisvilleLouisvilleUSA