Peptide De Novo Sequencing with MS/MS
Keywords and Synonyms
De novo sequencing ; Peptide sequencing
De novosequencing arises from the identification of peptides by using tandem mass spectrometry (MS/MS). A peptide is a sequence of amino acids in biochemistry and can be regarded as a string over a finite alphabet from a computer scientist's point of view. Each letter in the alphabet represents a different kind of amino acid, and is associate with a mass value. In the biochemical experiment, a tandem mass spectrometer is utilized to fragment many copies of the peptide into pieces, and to measure the mass values (in fact, the mass to charge ratios) of the fragments simultaneously. This gives a tandem mass spectrum. Since different peptides normally produce different spectra, it is possible, and now a common practice, to deduce the amino acid sequence of the peptide from its spectrum. Often this deduction involves the searching in a database for a peptide that can possibly produce the...
KeywordsTandem Mass Tandem Mass Spectrometry Score Function Dynamic Programming Algorithm Mass Spectrum Tandem