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Definition

Biopanning is the procedure of selecting binding partners from phage display libraries. In this affinity-driven selection procedure, specific binders against a target of choice are enriched from a phage display library during consecutive cycles of incubation, washing, amplification and re-selection of bound phage. Multiple rounds of selection are necessary to enrich specifically binding phage particles over a general background of unspecific sticky phage.

Protein Interaction Analysis: Phage Display

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© 2006 Springer-Verlag

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(2006). Biopanning. In: Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Springer, Berlin, Heidelberg . https://doi.org/10.1007/3-540-29623-9_6317

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