Cell Separation Techniques
Cell sorting, selection, isolation, enrichment, depletion.
This essay focuses on ex vivo and in vitro cell separation methods in the field of immunology and haematology. It does not include methods for in vivo depletion of certain cell types, for example by antibody treatment or in animal models-in particular in mice-by gene targeting.
ex vivo or in vitro analysis (e.g. for phenotypic, functional, biochemical or genetic analysis)
in vivo analysis (after adoptive transfer)
other purposes, like immunisation or generation of cell lines.
positive enrichment of target cells is the most direct way to isolate target cells and typically gives best purity and recovery; however, if positive selection is not feasible (e.g. because...