The Anaerobic Gram-Positive Cocci

  • Takayuki Ezaki
  • Na Li
  • Yoshiaki Kawamura


The anaerobic Gram-positive cocci (also known as peptococci and peptostreptococci) discussed in this chapter are limited to firmicutes with low mol% G+C. They are Peptococcus, Peptostreptococcus, Anaerococcus, Peptoniphilus, Gallicola, Finegoldia, Micromonas, Ruminococcus, Coprococcus and Sarcina. All except Anaerococcus, Peptoniphilus, Gallicola, Finegoldia and Micromonas belong to the classical family Peptococaceae (Rogosa, 1974). During the last 10 years, many members of the old genus Peptostreptococcus were transferred to many other genera. Most anaerobic Gram-positive cocci belong to the normal flora of the human gastrointestinal tract, vagina and oral cavity and often are found in human clinical specimens (Brook and Frazier, 1990; Ezaki et al., 1992). (Most human clinical isolates were identified as peptostreptococci.) Phylogenetically, anaerobic Gram-positive cocci belong to the phylum Clostridium. Indeed, based on 16S rRNA sequences, all species of the genus...


Vaginal Discharge Rumen Fluid Clostridial Cluster Fermentable Carbohydrate Phenylethyl Alcohol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Literature Cited

  1. Bartlett, J. G. 1990 Anaerobic cocci In: G. L. Mandell, R. G. Douglas, and J. E. Bennett (Eds.) The Principles and Practice of Infectious Diseases, 3rd ed Churchill Livingstone New York, NY 1867–1869Google Scholar
  2. Bartlett, J. G. 1993 Anaerobic bacterial infections of the lung and pleural space Clin. Infect. Dis. 16 (Suppl. 4)S248–S255PubMedCrossRefGoogle Scholar
  3. Bourgault, A. M., and J. E. Rosenblatt. 1979 First isolation of Peptococcus indolicus from a human clinical specimen J. Clin. Microbiol. 9 549–550PubMedGoogle Scholar
  4. Bourgault, A.-M., J. E. Rosenblatt, and R. H. Fitzgerald. 1980 Peptococcus magnus: A significant human pathogen Ann. Int. Med. 93 244–248PubMedGoogle Scholar
  5. Bowker, K. E., M. Wootton, H. A. Holt, D. S. Reeves, and A. P. MacGowan. 1996 The in-vitro activity of trovafloxacin and nine other antimicrobials against 413 anaerobic bacteria J. Antimicrob. Chemother. 38 271–281PubMedCrossRefGoogle Scholar
  6. British Society for Antimicrobial Chemotherapy. 1991 Guide to Sensitivity Testing Academic Press LondonGoogle Scholar
  7. Brook, I. 1981 Bacteriology of intracranial abscess in children J. Neurosurg. 54 484–488PubMedCrossRefGoogle Scholar
  8. Brook, I. 1988a Aerobic and anaerobic bacteriology of purulent nasopharyngitis in children J. Clin. Microbiol. 26 592–594PubMedGoogle Scholar
  9. Brook, I. 1988b Enhancement of growth of aerobic, anaerobic, and facultative bacteria in mixed infections with anaerobic and facultative Gram positive cocci J. Surg. Res. 45 222–227 26PubMedCrossRefGoogle Scholar
  10. Brook, I. 1988c Recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals J. Clin. Microbiol. 26 1181–1188 29PubMedGoogle Scholar
  11. Brook, I. 1989 Aerobic and anaerobic microbiology of Bartholin’s abscess Surg. Gynecol. Obstet. 169 32–34PubMedGoogle Scholar
  12. Brook, I., and E. H. Frazier. 1990 Aerobic and anaerobic bacteriology of wounds and cutaneous abscess Arch. Surg. 125 1445–1451PubMedCrossRefGoogle Scholar
  13. Brook, I. 1995 Anaerobic cocci In: G. L. Mandell, J. E. Bennett, and R. Dolin (Eds.) The Principles and Practice of Infectious Diseases, 4th ed Churchill Livingstone New York, NY 2204–2206Google Scholar
  14. Brook, I. 1996 Pyomyositis in children caused by anaerobic bacteria J. Pediatr. Surg. 31 394–396PubMedCrossRefGoogle Scholar
  15. Bryant, M. P. 1986 Genus Ruminococcus In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe and J. G. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 2 1093–l097Google Scholar
  16. Canale-Parola, E. 1970 Biology of the sugar-fermenting sarcinae Bacteriol. Rev. 34 82–97PubMedGoogle Scholar
  17. Canale-Parola, E. 1986 Genus Sarcina In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 2 1100–1103Google Scholar
  18. Carlsson, J., J. T. Larsen, and M.-B. Edlund. 1993 Peptostreptococcus micros has a uniquely high capacity to form hydrogen sulfide from glutathione Oral Microbiol. Immunol. 8 42–45PubMedCrossRefGoogle Scholar
  19. Christensen, H., O. Angen, R. Mutters, J. E. Olsen, and M. Bisgaard. 2000 DNA-DNA hybrdization determined in micro-wells using covalent attachment of DNA Int. J. Syst. Evol. Microbiol. 50 1095–1102PubMedCrossRefGoogle Scholar
  20. Citron, D. M., E. J. C. Goldstein, M. A. Kenner, L. B. Burnham, and C. B. Inderlied. 1995 Activity of ampicillin/sulbactam, ticarcillin/clavulanae. clarithromycin, and eleven other antimicrobial agents against anaerobic bacteria isolated from infections in children Clin. Infect. Dis. 20 (Suppl. 2)S356–S360PubMedCrossRefGoogle Scholar
  21. Collins, M. D., P. A. Lawson, A. Willems, J. J, Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow. 1994 The phylogeny of the genus Clostridium: Proposal of five new genera and eleven new species combinations Int. J. Syst. Bacteriol. 44 812–826PubMedCrossRefGoogle Scholar
  22. Crowther, J. S. 1971 Sarcina ventriculi in human feces J. Med. Microbiol. 4 343–349PubMedCrossRefGoogle Scholar
  23. Evans, C. A., K. L. Mattern, and S. L. Hallam. 1978 Isolation and identification of Peptococcus saccharolyticus from human skin J. Clin. Microbiol. 7 261–264PubMedGoogle Scholar
  24. Ezaki, T., H. Yamamoto, K. Ninomiya, S. Suziki, and E. Yabuuchi. 1983 Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii, and Peptococcus magnus to the genus Peptostreptococcus and proposal of Peptostreptococcus tetradius sp. nov. Int. J. Syst. Bacteriol. 33 683–698CrossRefGoogle Scholar
  25. Ezaki, T., and E. Yabuuchi. 1985 Oligopeptidase activity of Gram-positive anaerobic cocci uscd for rapid identification J. Gen. Appl. Microbiol. 31 255–266CrossRefGoogle Scholar
  26. Ezaki, T., Y. Hashimoto, and E. Yabuuchi. 1989 Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains Int. J. Syst. Bacteriol. 39 224–229CrossRefGoogle Scholar
  27. Ezaki, T., S.-L. Liu, Y. Hashimoto, and E. Yabuuchi. 1990 Peptostreptococcus hydrogenalis sp. nov. from human fecal and vaginal flora Int. J. Syst. Bactcriol. 40 305–306CrossRefGoogle Scholar
  28. Ezaki, T., H. Oyaizu, and E. Yabuuchi. 1992 The anaerobic Gram-positive cocci In: A. Balows, H. G. Truper, M. Dworkin, W. Harder, and K. H. Schleifer (Eds.) [{}{The Prokaryotes, 2nd ed.]} Springer-Verlag Berlin 1879–1892Google Scholar
  29. Ezaki, T., N. Li, Y. Hashimoto, H. Miura, and H. Yamamoto. 1994 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov Int. J. Syst. Bacteriol. 44 130–136PubMedCrossRefGoogle Scholar
  30. Ezaki, T., Y. Kawamura, N. Li, Z. Y. Li, L. Zhao, and S. Shu. 2000 Proposal of Genera Anaerococus gen. nov., Peptoniphilus gen. nov., and Gallicola gen. nov. for members of Genus Peptostreptococcus Int. J. Syst. Evol. Microbiol.Google Scholar
  31. Finegold, S. M., H. R. Attebery, and V. L. Sutter. 1974 Effect of diet on human fecal flora: Comparison of Japanese and American diets Am. J. Clin. Nutr. 27 1456–1469PubMedGoogle Scholar
  32. Finegold, S. M., and NCCLS Working Group on Anaerobic Susceptibility Testing. 1988 Susceptibility testing of anaerobic bacteria J. Clin. Microbiol. 26 1253–1256PubMedGoogle Scholar
  33. Finegold, S. M., and W. L. George. 1989 Anaerobic Infections in Humans Academic Press New York, NYGoogle Scholar
  34. Finegold, S. M. 1995 Anaerobic infections in humans: An overview Anaerobe l 3–9CrossRefGoogle Scholar
  35. Foubert, E. L., and H. C. Douglas. 1948 Studies on the anaerobic micrococci. l: Taxonomic considerations J. Bacteriol. 56 25–34Google Scholar
  36. Fuente, R., G. Suarez, and K. H. Schleifer. 1985 Staphylococcus aureus subsp. anaerobius subsp. nov., the causal agent of abscess disease of sheep Int. J. Syst. Bacteriol. 35 99–102CrossRefGoogle Scholar
  37. Greenwood, D., and J. Palfreyman. 1987 Comparative activity of LY146032 against anaerobic cocci Eur. J. Clin. Microbiol. Infect. Dis. 6 682–684CrossRefGoogle Scholar
  38. Hall, I. C. 1930 Micrococcus niger, a new pigment forming anaerobic coccus recovered from urine in a case of general arteriosclerosis J. Bacteriol. 20 407–415PubMedGoogle Scholar
  39. Hill, G. B., K. K. St. Claire, and L. T. Gutman. 1995 Anaerobes predominate among the vaginal microflora of prepubertal girls Clin. Infect. Dis. 20 (Suppl. 2)S269–S270PubMedCrossRefGoogle Scholar
  40. Hillier, S. L., and B. J. Moncla. 1991 Anaerobic Gram-positive cocci In: A. Balows, W. J. Hausler, K. L. Herrmann. H. D. Isenberg, and H. J. Shadomy (Eds.) Manual of Clinical Microbiology, 5th ed American Society for Microbiology Washington DC 533–537Google Scholar
  41. Hillier, S. L., M. A. Krohn, L. K. Rabe, S. J. Klebanoff, and D. A. Eschenbach. 1993 The normal vaginal flora, H2O2-producing lactobacilli, and bacterial vaginosis in pregnant women Clin. Infect. Dis. 16 (Suppl. 4)S273–S281PubMedCrossRefGoogle Scholar
  42. Hoi-Sorenson, G. 1973 Micrococcus indolicus: Some biochcmical properties, and the demonstration of six antigenically different types Acta Vet. Scand. 14 301–326Google Scholar
  43. Holdeman, L. V., and W. E. C. Moore. 1974 New genus, Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human feces Int. J. Syst. Bacteriol. 24 26–277CrossRefGoogle Scholar
  44. Holdeman, L. V., E. P. Cato, and W. E. C. Moore. 1977 Anaerobe Laboratory Manual, 4th ed Anaerobe Laboratory, Virginia Polytechnic Institute and State University Blacksburg, VAGoogle Scholar
  45. Holdeman Moore, L. V., and W. E. C. Moore. 1986a Genus Coprococcus In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 2 1097–l099Google Scholar
  46. Holdeman Moore, L. V., J. L. Johnson, and W. E. C. Moore. 1986b Genus Peptococcus In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 2 1082–1083Google Scholar
  47. Holdeman Moore, L. V., J. L. Johnson, and W. E. C. Moore. 1986c Genus Peptostreptococcus In: P. H. A. Sneath, N. S. Mair, M. E. Sharpe, and J. G. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 2 1083–1092Google Scholar
  48. Hungate, R. E. 1966 The rumen and its microbes Academic Press New York, NYGoogle Scholar
  49. Hunter, T., and A. W. Chow. 1988 Peptostreptococcus magnus septic arthritis: A report and review of the English literature J. Rheumatol. 15 1583–1584PubMedGoogle Scholar
  50. Johnson J. L. 1984 Nucleic acids in bacterial classification In: N. R. Krieg, and J. H. Holt (Eds.) [{}Bergey’s Manual of Systematic Bacteriology] Williams & Wilkins Baltimore, MD 1 8–11Google Scholar
  51. Johnson, C. C. 1993 Susceptibility of anaerobic bacteria to β-lactam antibiotics in the United States Clin. Infect. Dis. 16 (Suppl. 4)S371–S376PubMedCrossRefGoogle Scholar
  52. Johnson, S., F. Lebahn, L. R. Peterson, and D. N. Gerding. 1995 Use of an anaerobic collection and transport swab device to recover anaerobic bacteria from infected foot ulcers in diabetics Clin. Infect. Dis. 20 (Suppl. 2)S289–S290PubMedCrossRefGoogle Scholar
  53. Kilpper-Baelz, R., and K. H. Schleifer. 1981 Transfer of Peptococcus saccharolyticus Foubert and Douglas to the genus Staphylococcus: Staphylococcus saccharolyticus (Foubert and Douglas) comb. nov Zentralbl. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. I, Orig. Reihe C2 324–331Google Scholar
  54. Kilpper-Baelz, R., and K. H. Schleifer. 1988 Transfer of Streptococcus morbillorum to the genus Gemella as Gemella morbillorum comb. nov. Int. J. Syst. Bacteriol. 38 442–443CrossRefGoogle Scholar
  55. Li, N., Y. Hashimoto, S. Adnan, H. Miura, H. Yamamoto, and T. Ezaki. 1992 Three new species of the genus Peptostreptococcus from humans: Peptostreptococcus vaginalis sp. nov., Peptostreptococcus lacrimalis sp. nov. and Peptostreptococcus !actolyticus sp. nov Int. J. Syst. Bactcriol. 42 602–605CrossRefGoogle Scholar
  56. Li, N., Y. Hashimoto, and T. Ezaki. 1994 Determination of l6S ribosomal RNA sequences of all members of the genus Peptostreptococcus and their phylogenetic position FEMS Microbiol. Lett. 116 1–6PubMedCrossRefGoogle Scholar
  57. Madsen, M., B. Aalbaek, and J. W. Hansen. 1992 Comparative bacteriological studies on summer mastitis in grazing cattle and pyogenes mastitis in stabled cattle in Denmark Vet. Microbiol. 32 81–88PubMedCrossRefGoogle Scholar
  58. Marui, T. 1981 Characterization of peptococci and peptostreptococci jsolated from human clinical specimens and their taxonomical problems Acta Sch. Med. Univ. Gifu 29 1071–l082Google Scholar
  59. Moore, W. E. C., and L. V. Holdeman. 1974 Human fecal flora: The normal flora of 20 Japanese-Hawaiians Appl. Microbiol. 27 961–979PubMedGoogle Scholar
  60. Moore, L. V. H., W. E. C. Moore, E. P. Cato, R. M. Smibert, J. A. Burmeister, A. M. Best, and R. R. Ranney. 1987 Bacteriology of human gingivitis J. Dent. Res. 66 989–995PubMedCrossRefGoogle Scholar
  61. Murdoch, D. A., I. J. Mitchelmore, and S. Tabaqchali. 1988 Peptostreptococcus micros in polymicrobial abscesses Lancet 1 594PubMedCrossRefGoogle Scholar
  62. Murdoch, D. A., and I. J. Mitchelmore. 1991 The laboratory identification of Gram-positive anaerobic cocci J. Med. Microbiol. 34 295–308PubMedCrossRefGoogle Scholar
  63. Murdoch, D. A., M. D. Collins, A. Willems, J. M. Hardie, K. A. Young, and J. T. Magee. 1997 Description of three new species of the genus Peptostreptococcus from human clinical specimens: Peptostreptococcus harei sp. nov., Peptostreptococcus ivorii sp. nov. and Peptostreptococcus octavius sp. nov Int. J. Syst. Bacteriol. 47 781–787CrossRefGoogle Scholar
  64. Murdoch, D. A. 1998 Gram-positive anaerobic cocci Clin. Microbiol. Rev. 11 81–120PubMedGoogle Scholar
  65. Murdoch, D. A., and H. N. Shah. 1999 Reclassification of Peptostreptococcus magnus (Prevot 1933)Holdeman and Moore 1972 as Finegoldia magna comb. nov. and Peptostreptococcus micros (Prevot 1933) Smith 1957 as Micromonas micros comb. nov Anaerobe 5 555–559CrossRefGoogle Scholar
  66. National Committee for Clinical Laboratory Standards. 1990 Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 2nd ed.: Approved Standard Publication M1 l-A2 National Committee for Clinical Laboratory Standards Villanova, PAGoogle Scholar
  67. Panichi, G., R. di Rosa, P. Enrico, and S. Babudieri. 1990 Anaerobic bacteria and bacterial infections: Perspectives on treatment and resistance in Italy Rev. Infect. Dis. 12 (Suppl. 2)S152–S156PubMedCrossRefGoogle Scholar
  68. Rainey, F. A., and P. H. Janssen. 1995 Phylogenetic analysis by 16S ribosomal DNA sequence comparison reveals two unrelated groups of species within the genus Ruminococcus FEMS Microbiol. Lett. 129 69–74PubMedGoogle Scholar
  69. Rogosa, M. 1971 Peptococcaceae, a new family to include the grampositive, anaerobic cocci of the genera Peptococcus, Peptostreptococcus. and Ruminococcus Int. J. Syst. Bacteriol. 21 234–237CrossRefGoogle Scholar
  70. Rogosa, M. 1974 Family III: Peptococcaceae In: R. E. Buchanan and N. E. Gibbons (Eds.) [{}Bergey’s Manual of Determinative Bacteriology, 8th ed.] Williams & Wilkins Baltimore, MD 517–527Google Scholar
  71. Sanchez, M. L., R. N. Jones, and J. L. Croco. 1992 Use of the E-Test to assess macrolide-lincosamide resistance patterns among Peptostreptococcus species Antimicrob. Newsl. 8 45–49CrossRefGoogle Scholar
  72. Sanderson, P. J. 1977 Infection of the foot with Peptococcus magnus J. Clin. Pathol. 30 266–268PubMedCrossRefGoogle Scholar
  73. Schiefer-Ullrich, H., and J. R. Andreesen. 1985 Peptostreptococcus barnesae sp. nov., a Gram-positive, anaerobic, obligately purine-utilising coccus from chicken feces Arch. Microbiol. 143 26–31CrossRefGoogle Scholar
  74. Shah, H. N., and S. E. Gharbia. 1995 The biochemical milieu of the host in the selection of anaerobic species in the oral cavity Clin. Infect. Dis. 20 (Suppl. 2)S291–S300PubMedCrossRefGoogle Scholar
  75. Shatha, A., N. Li, H. Miura, Y. Hashimoto, Y. Yamamoto, and T. Ezaki. 1993 Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours FEMS Microbiol. Lett. 106 139–142CrossRefGoogle Scholar
  76. Skerman, V. B. D., V. MacGowan, and P. H. A. Sneath. 1980 Approved lists of bacterial names Int. J. Syst. Bacteriol. 30 225–240CrossRefGoogle Scholar
  77. Stackebrandt, E., and B. M. Goebel. 1994a Taxonomic note: A place for DNA/DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology Int. J. Syst. Bacteriol. 44 846–849CrossRefGoogle Scholar
  78. Stackebrandt, E., and W. Liesack. 1994b Nucleic acids and classification In: M. Goodfellow and A. G. O’Donnnell (Eds.) Handbook of New Bacterial Systematics Academic Press London 152–189Google Scholar
  79. Tanner, A., and N. Stillman. 1993 Oral and dental infections with anaerobic bacteria: Clinical features, predominant pathogens, and treatment Clin. Infect. Dis. 16 (Suppl. 4)S304–S309PubMedCrossRefGoogle Scholar
  80. Thomas, C. G. A., and R. Hare. 1954 The classification of anaerobic cocci and their isolation in normal human beings and pathological processes J. Clin. Pathol. 7 300–304PubMedCrossRefGoogle Scholar
  81. Thompson J. D. 1994 Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucl. Acids Res. 22 4673–4680PubMedCrossRefGoogle Scholar
  82. Van Dalen, P. J., T. J. M. van Steenbergen, M. M. Cowan, H. J. Busscher, and J. de Graaff. 1993 Description of two morphotypes of Peptostreptococcus micros Int. J. Syst. Bacteriol. 43 787–793PubMedCrossRefGoogle Scholar
  83. Wade, W. G., J. Downes, D. Dymock, S. J. Hiom, A. J. Weightman, F. E. Dewhirst, B. J. Paster, N. Tzellas, and B. Coleman. 1999 The family Coriobacteriaceae: Reclassification of Eubacterium exiguum (Poco et al., 1996) and Peptostreptococcus heliotrinreducens (Lanigan, 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot, 1938) as Eggerthella lenta gen. nov., comb. nov Int. J. Syst. Bacteriol. 49 595–600PubMedCrossRefGoogle Scholar
  84. Wayne, L. G., D. J. Brenner, R. R. Colwell, et al. 1987 Report of the ad hoc committee on reconcilation of approaches to bacterial systematics Int. J. Syst. Bacteriol. 37 463–464CrossRefGoogle Scholar
  85. Wilkins, T. D., W. E. C. Moore, S. E. H. West, and L. V. Holdeman. 1975 Peptococcus niger (Hall) Kluyver and van Niel 1936: Emendation of description and designation of neotype strain Int. J. Syst. Bacteriol. 25 47–49CrossRefGoogle Scholar
  86. Wilkins, T. D., and S. Chalgren. 1976 Medium for use in antibiotic susceptibility testing of anaerobic bacteria Antimicrob. Agents Chemother. 10 926–928PubMedGoogle Scholar
  87. Willems, A., and M. D. Collins. 1994 Phylogenetic placement of Sarcina ventriculi and Sarcina maxima within Group I Clostridium, a possible problem for future revision of the genus Clostridium: Request for an opinion Int. J. Syst. Bacteriol. 44 591–593PubMedCrossRefGoogle Scholar
  88. Willems, A., and M. D. Collins. 1995 Phylogenetic analysis of Ruminococcus flavefaciens, the type species of the genus Ruminococcus, does not support the reclassification of Streptococcus hansenii and Peptostreptococcus productus as ruminococci Int. J. Syst. Bacteriol. 45 572–575PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Takayuki Ezaki
  • Na Li
  • Yoshiaki Kawamura

There are no affiliations available

Personalised recommendations