Encyclopedia of Lipidomics

Living Edition
| Editors: Markus R. Wenk

Derivatization in Gas Chromatography of Lipids

  • Tomáš Řezanka
  • Karolína Pádrová
  • Karel Sigler
Living reference work entry
DOI: https://doi.org/10.1007/978-94-007-7864-1_71-1
Derivatization is performed as part of gas chromatographic (GC) analysis of lipids primarily to extend the spectrum of substances that can be determined by this method. Derivatization in general converts less volatile and thermally labile substances into compounds that can be analyzed in the gaseous state. One of the most important methods is protection (derivatization) of polar groups such as –OH, –NH 2, –SH, or –COOH. Due to an elevated temperature in a gas chromatograph, the analysis of compounds having free polar groups either suffers from a poor division (formation of tailing peaks) like, e.g., in the case of free fatty acids, or the analysis completely fails, e.g., in the case of saccharides formed after the hydrolysis of glycopeptides (e.g., digalactosyldiacylglycerols) (Table 1).
Table 1

Names of conventional derivatizing agents

Method

Reagent

Common name

Silylation

BSA

Bistrimethylsilylacetamide

 

BSTFA

Bistrimethylsilyltrifluoroacetamide

 

HMDS

Hexamethyldisilane

 

MSTFA

N-methyl-trimethylsilyltrifluoroacetamide...

Keywords

Methyl Ester Fatty Acid Methyl Ester Dimethyl Disulfide Silyl Ether Trifluoroacetic Anhydride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Ahuja S. Derivatization in gas chromatography. J Pharm Sci. 1976;65:163–82.CrossRefPubMedGoogle Scholar
  2. Beneytout J, Tixier M, Rigaud M. Capillary gas – liquid or thin-layer chromatographic resolution of 2-hydroxy-fatty acid enantiomers. J Chromatogr A. 1986;351:363–5.CrossRefGoogle Scholar
  3. Biermann CJ, McGinnis GD. Analysis of carbohydrates by GLC and MS. Boca Raton: CRC Press; 1988.Google Scholar
  4. Blau K, King GS. Handbook of derivatives for chromatography. London: Heyden & Sons Ltd; 1979.Google Scholar
  5. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959;37:911–7.CrossRefPubMedGoogle Scholar
  6. Brenna JT. Structural analysis of unsaturated fatty acid methyl ester isomers with acetonitrile covalent adduct chemical ionization (CACI) tandem mass spectrometry. In: Mossoba MM, Kramer JKG, Brenna JT, McDonald RE, editors. Lipids analysis and lipidomics: new techniques and applications. Champaign: AOCS Press; 2006. p. 157–72.CrossRefGoogle Scholar
  7. Christie WW. Gas chromatography and lipids: a practical guide. Dundee: The Oily Press; 1989.Google Scholar
  8. Christie WW. Gas chromatography–mass spectrometry methods for structural analysis of fatty acids. Lipids. 1998;33:343–53.CrossRefPubMedGoogle Scholar
  9. Christie W, Han X. Lipid analysis: isolation, separation, identification and lipidomic analysis. 4th ed. Bridgwater: The Oily Press; 2010.CrossRefGoogle Scholar
  10. Christie WW, Stefanov K. Separation of picolinyl ester derivatives of fatty-acids by high-performance liquid-chromatography for identification by mass-spectrometry. J Chromatogr. 1987;392:259–65.CrossRefGoogle Scholar
  11. Harvey D. Mass spectrometry of picolinyl and other nitrogen-containing derivatives of lipids. In: Chritie W, editor. Advances in lipid methodology – one. Ayr: The Oily Press; 1992. p. 19–80.Google Scholar
  12. Knapp DR. Handbook of analytical derivatization reactions. New York: Wiley-Interscience; 1979.Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2016

Authors and Affiliations

  • Tomáš Řezanka
    • 1
  • Karolína Pádrová
    • 2
  • Karel Sigler
    • 1
  1. 1.Institute of Microbiology, CASPragueCzech Republic
  2. 2.Department of BiotechnologyUniversity of Chemical Technology PraguePragueCzech Republic