Structural Elucidation of Glycerolipids (TGs, GPLs) by Vacuum MALDI HE-CID TOF/RTOF Mass Spectrometry
In terms of mass spectrometric analysis, glycerolipids (GLs) can be divided into two major compound classes, triacylglycerols (TGs) and glycerophospholipids (GPLs). The latter group can be subdivided into glycerophosphatidic acids (GPA), glycerophosphatidylserine (GPS), glycerophosphatidylethanolamine (GPE), glycerophosphatidylcholine (GPC), glycerophosphatidylglycerol (GPG), glycerophosphatidylinositol (GPI), and the dimeric GPL cardiolipin. Under vacuum MALDI conditions, glycerolipids form either [M-H]−, [M + H]+, or various types of (multiply) sodiated species (even without the addition of sodium salts) depending on their particular structure. For structural elucidation high-energy collision-induced dissociation in combination with tandem time-of-flight mass spectrometry (HE-CID TOF/RTOF-MS) at high collision energies (ELAB = 20 keV) offers the most complete fragmentation across the recorded m/z range in one step of all tandem mass spectrometric techniques currently available.
KeywordsPolar Head Group Fatty Acid Substituent Charge Remote Fragmentation Fatty Acid Anion Choline Phosphate
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