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Heterologous Glycoprotein Production (Yeast)

  • Yasunori ChibaEmail author
Reference work entry

Abstract

Biologics has attracted a great deal of attention, and antibodies, enzymes, cytokines, and vaccines for therapeutic uses are being developed. These proteins are mostly glycosylated and therefore are produced in mammalian cells. From the viewpoint of cost reduction and risk management of viral infection spreading through the use of contaminated protein therapeutics, the development of alternative glycoprotein expression system has been promoted, and consequently, protein expression systems in yeast have been expected. Yeast cells cannot generate human-type glycans, and the different glycan structures on the therapeutic glycoproteins produced in yeast may cause immunological incompatibility with human. Glycan remodeling of yeast cells has been one of the issues in order to produce heterologous glycoprotein, and recent advances in the field of yeast glycobiology and synthetic biology have shown significant promise. In this chapter, several significant developments in this field are reviewed.

Keywords

Yeast Glycoprotein Glycan remodeling N-glycan Mannose-6-phosphate O-glycan 

References

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Copyright information

© Springer Japan 2015

Authors and Affiliations

  1. 1.Glycomedicine Technology Research CenterNational Institute of Advanced Industrial Science and Technology (AIST)TsukubaJapan

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