Purification Method of Drug-Loaded Liposome

  • Meng Lin
  • Xian-Rong QiEmail author
Living reference work entry
Part of the Biomaterial Engineering book series (BIOENG)


Liposomes are lipid-based bilayer vesicles which can encapsulate drugs to improve their pharmacokinetic performance and thus are widely used as drug delivery system. Drugs loaded in liposomes have distinct pharmacokinetic characteristics from non-encapsulated drugs. Thus, the purification of liposomes, i.e., separating or removing nonencapsulated drugs as well as detergent used in the preparation process, is important for quality control of liposomal products. From a pharmaceutical point of view, well-defined liposome preparations should use well-characterized lipids and would avoid the use of organic solvents and detergents (which are difficult to remove). In this chapter, we reviewed some methods for liposome purification, including dialysis, column chromatographic separation method, centrifugation, protamine aggregation method, ion-exchange resin, and ultrafiltration method. For each method, its basic principle and characteristics are introduced, and some examples are also given. To achieve effective purification, suitable methods should be chosen based on the characteristics of every individual liposome and optimization of separation condition is also demanded. And more efforts needed to be put into the liposome purification field to improve these methods as well as develop other new techniques with a wide range of application.


Liposomes Purification Dialysis Column chromatography Centrifugation Protamine aggregation method Ion-exchange resin Ultrafiltration 


  1. Assanhou AG, Li W, Zhang L, Xue LJ, Kong LY, Sun HB, Mo R, Zhang C (2015) Reversal of multidrug resistance by co-delivery of paclitaxel and lonidamine using a TPGS and hyaluronic acid dual-functionalized liposome for cancer treatment. Biomaterials 73:284–295CrossRefGoogle Scholar
  2. Calle D, Negri V, Ballesteros P, Cerdan S (2015) Magnetoliposomes loaded with poly-unsaturated fatty acids as novel theranostic anti-inflammatory formulations. Theranostics 5(5):489–503CrossRefGoogle Scholar
  3. Chen JT, Pan H, Yang YN, Xiong SH, Duan HL, Yang XG, Pan WS (2018) Self-assembled liposome from multi-layered fibrous mucoadhesive membrane for buccal delivery of drugs having high first-pass metabolism. Int J Pharm 547(1–2):303–314CrossRefGoogle Scholar
  4. Dario C, Elisabetta B, Joshua O, Eleanor S, Claudio N (2016) Liposome production by microfluidics: potential and limiting factors. Sci Rep 6:25876CrossRefGoogle Scholar
  5. Dimov N, Kastner E, Hussain M, Perrie Y, Szita N (2017) Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. Sci Rep 7(1):12045–12057CrossRefGoogle Scholar
  6. Dipali SR, Kulkarni SB, Betageri GV (1996) Comparative study of separation of non-encapsulated drug from unilamellar liposomes by various methods. J Pharm Pharmacol 48(11):1112CrossRefGoogle Scholar
  7. Jin YG, Du LN, Chen Y, Ling PX (2015) Application of nanotechnology in drug delivery, 1st edn, Chemical Industry Press, Beijing, pp 11–12, pp 175–177Google Scholar
  8. Khatri N, Baradia D, Vhora I, Rathi M, Misra A (2014) cRGD grafted liposomes containing inorganic nano-precipitate complexed siRNA for intracellular delivery in cancer cells. J Control Release 182(10):45–57CrossRefGoogle Scholar
  9. Li HR, Li SF (2007) The measurement of the entrapment efficiency of drugs in liposome. Chin J Pharm Anal 27(11):1844–1848Google Scholar
  10. Torchilin VP, Weissing V (2002) Liposomes, 2nd edn, Oxford University Press, New York, pp 45–46, pp 149–164Google Scholar
  11. Yang ZZ, Zhang YQ, Wang ZZ, Wu K, Lou JN, Qi XR (2013) Enhanced brain distribution and pharmacodynamics of rivastigmine by liposomes following intranasal administration. Int J Pharm 452(1–2):344–354CrossRefGoogle Scholar
  12. Yang WT, Hu Q, Xu YM, Liu HL, Zhong L (2018) Antibody fragment-conjugated gemcitabine and paclitaxel-based liposome for effective therapeutic efficacy in pancreatic cancer. Mater Sci Eng C-Mater 89:328–335CrossRefGoogle Scholar
  13. Yoon HY, Kwak SS, Jang MH, Kang MH, Sung SW, Kim CH, Kim SR, Yeom DW, Kang MJ, Choi YW (2017) Docetaxel-loaded RIPL peptide (IPLVVPLRRRRRRRRC)-conjugated liposomes: drug release, cytotoxicity, and antitumor efficacy. Int J Pharm 523(1):229–237CrossRefGoogle Scholar

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© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Natural and Biomimetic Drugs, Beijing Key Laboratory of Molecular Pharmaceutics and New Drug System, School of Pharmaceutical SciencesPeking UniversityBeijingChina

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