Encyclopedia of Parasitology

2016 Edition
| Editors: Heinz Mehlhorn

Sedimentation

  • Heinz MehlhornEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-3-662-43978-4_4317
This is a method to isolate larger eggs of trematodes (more than 50–60 μm in length) (e.g., those of Fasciola,  Schistosoma), of nematodes (e.g., Ascaris), of  Acanthocephala and  Pentastomida, while smaller-sized eggs (smaller than 50 μm) or even oocysts of coccidians will only be poorly determined by this method, if larger numbers occur. In these cases, other concentration methods have to be used, e.g.,  S.A.F.C.,  M.I.F.C., and/or  flotation. The sedimentation method uses the following steps:
  1. 1.

    5–10 g feces are mixed with 100 ml tap water (for Schistosoma-suspected feces use a physiological saline, which avoids hatching of miracidium larvae).

     
  2. 2.

    Larger particles are extracted by sieving the fluid.

     
  3. 3.

    The remnant solution is filled into a glass cylinder and left to settle for about 30 min.

     
  4. 4.

    Afterward, the supernatant fluid is slowly decanted and filled up with fresh water respectively saline.

     
  5. 5.

    This process is repeated until the supernatant fluid appears widely clear.

     
  6. 6.
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Futher Reading

  1. Mehlhorn H (2016) The parasites of animals, 8th edn. Springer Spektrum, HeidelbergCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany