Encyclopedia of Parasitology

2016 Edition
| Editors: Heinz Mehlhorn

SAFC Concentration

  • Heinz MehlhornEmail author
Reference work entry
DOI: https://doi.org/10.1007/978-3-662-43978-4_4310
This is a method to isolate parasitic stages from feces respectively stools of animals or humans by help of a so-called sodium acetate-acetic acid-formalin solution.
  1. 1.

    Stool sample (1 g) is placed into a tube containing 10 ml of S.A.F.

     
  2. 2.

    Stool and S.A.F. are intensively mixed by a glass stick.

     
  3. 3.

    Closed tube is shaken vigorously.

     
  4. 4.

    Stool is filtered using a funnel with gauze.

     
  5. 5.

    The tubes are now centrifuged for 1 minute at 2,000 rpm.

     
  6. 6.

    Supernatant is removed by help of a pipette or cautiously decanted.

     
  7. 7.

    7 ml saline is added and contents are mixed with a glass stick.

     
  8. 8.

    3 ml ether has to be added.

     
  9. 9.

    Tubes are closed with rubber stoppers, well shaken, while fixing firmly the stopper (or closing the vessel by a cover).

     
  10. 10.

    The cover (or rubber stopper) is removed and the product is centrifuged for 5 min at 2,000 rpm.

     
  11. 11.

    After centrifugation all three upper layers were decanted and just the sediment is retained and investigated by help of a light microscope...

This is a preview of subscription content, log in to check access.

Further Readings

  1. Blagg W et al (1955) New concentration technique for demonstration of protozoan and helminth eggs in faeces. Am J Trop Meg Hyg 4:23–28Google Scholar
  2. Marti H, Escher E (1980) SAF – an alternative fixation solution for parasitological stool specimens. Schweiz Med Wochenschr 120:1473–1476Google Scholar
  3. Mehlhorn H et al (1992) Diagnose und Therapie der Parasiten von Haus-, Nutz- und Heimtieren, 2nd edn. Gustav Fischer Verlag, Stuttgart/New YorkGoogle Scholar
  4. Price LD (1981) Comparison of three collection-preservation methods for detection of intestinal parasites. J Clin Microbiol 14:656–660PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Institut für Zoomorphologie, Zellbiologie und ParasitologieHeinrich-Heine-UniversitätDüsseldorfGermany