Encyclopedia of Parasitology

2016 Edition
| Editors: Heinz Mehlhorn

Enzyme-Linked Immunosorbent Assay (ELISA)

Reference work entry
DOI: https://doi.org/10.1007/978-3-662-43978-4_1077

This is a type of sandwich-test for the diagnosis (e.g., of parasites) using antigen or antibodies that were marked with enzymes. In case of antigen-ELISA a specific antibody (directed against the antigen) becomes attached at a plate. Then the test substance (with the antigen) is filled onto the plate thus giving rise to antibody-antigen-complexes. As next step a second antigen specific antibody, which is marked with an enzyme, is added. If then the test substrate as added, the antigen can become qualitatively and quantitatively determined.

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© Springer-Verlag Berlin Heidelberg 2016