Local Lymph Node Assay (IMDS), Modifications
In 1998, after a decade of scientific evaluation and extensive interlaboratory validation, the local lymph node assay (see Local Lymph Node Assay) was peer reviewed and was, in principle, endorsed as a stand-alone test by an independent panel of experts on behalf of the US Interagency Co-ordinating Committee on the Validation of Alternative Methods (ICCVAM) in 1999. However, improvements were suggested by the peer review panel concerning the discrimination of contact allergenic from irritation potential. Some irritating and phototoxic compounds were shown to cause a false-positive response, since they induced a vigorous cell proliferation in the ear-draining lymph nodes (Scholes et al. 1992) or a marked LN hyperplasia (Homey et al. 1998; Ulrich et al. 1998). The occurrence of false-positive results is an obvious consequence of the tight physiological connection between inflammatory tissue processes and specific immune reactions, with the latter being more vigorous, when...
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