Local Lymph Node Assay
The murine local lymph node assay (LLNA) was developed initially as a method for the identification of chemicals that have the potential to cause skin sensitization and allergic contact dermatitis. Since its first description the LLNA has been the subject of detailed assessments in the context of national and international collaborative trials and of extensive comparisons with both other predictive test methods and human data. The origins, development, evaluation, and eventual validation of the LLNA have recently been described comprehensively elsewhere (Kimber et al. 2002a).
In contrast to guinea pig tests, the LLNA identifies contact allergens as a function of events induced during the induction phase of skin sensitization – specifically the stimulation of proliferative responses in draining lymph nodes.
The LLNA is based on an appreciation of the events that characterize the induction phase of skin sensitization. Following topical exposure to a...
- Gerberick GF, Robinson MK, Ryan CA et al (2001) Contact allergenic potency: correlation of human and local lymph node assay data. Am J Cont Derm 12:156–161Google Scholar