Encyclopedia of Biophysics

Living Edition
| Editors: Gordon Roberts, Anthony Watts, European Biophysical Societies

Techniques Applied to Glycan Structure and Conformation

  • Elizabeth Hounsell
Living reference work entry
DOI: https://doi.org/10.1007/978-3-642-35943-9_99-1

Synonyms

Definition

The techniques applied to glycan/oligosaccharide/polysaccharide structure and conformation are the same as those used to analyze the protein to which they can be attached (Glycoproteins), but with specific adaptations that have been honed over the years to adapt to the different functional groups of oligosaccharides, their diversity of monosaccharide type, linkages, and branching and anomericity. Monosaccharide analysis methods, mass spectrometry, circular dichroism (Carbohydrate Circular Dichroism), NMR (Carbohydrate NMR Spectroscopy) and X-ray crystallography (X-Ray Diffraction and Crystallography of Oligosaccharides and Polysaccharides), and different forms of microscopy all give clues to parts of the structural and conformational puzzle. In some cases, analysis of intact glycoproteins can be carried out. In others, the different biophysical...

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References

  1. Hounsell EF (1994) Physicochemical analysis of oligosaccharide determinants of glycoproteins. Adv Carbohydr Chem Biochem 50:311–350CrossRefPubMedGoogle Scholar
  2. Hounsell EF (1995) 1H NMR in the structural and conformational analysis of oligosaccharides and glycoconjugates. Prog Nucl Magn Reson 27:445–474CrossRefGoogle Scholar
  3. Hounsell EF (1997) Methods of glycoconjugate analysis. In: Gabius H-J, Gabius S (eds) Glycosciences. Chapman and Hall, Weinheim, pp 15–29Google Scholar
  4. Hounsell EF (ed) (1998) Glycoanalysis protocols, Methods in molecular biology, vol 76, 2nd edn. Humana Press, TotowaGoogle Scholar
  5. Hounsell EF (2010) NMR of carbohydrates, lipids and membranes. In: Webb GA (ed) RSC specialist reports in NMR spectroscopy, vol 39. Royal Society of Chemistry Press, Cambridge, pp 290–320. (Previous Vols 29–38)Google Scholar
  6. Hounsell EF, Bailey D (1997) Approaches to the structural determination of oligosaccharides and glycopeptides by NMR. In: Large DG, Warren CD (eds) Glycopeptides and related compounds. Marcel Dekker, New York, pp 631–660Google Scholar
  7. Sinnott ML (2007) Carbohydrate chemistry and biochemistry: structure and metabolism. RSC, CambridgeGoogle Scholar

Copyright information

© European Biophysical Societies' Association (EBSA) 2018

Authors and Affiliations

  1. 1.School of Biological and Chemical SciencesBirkbeck College, University of LondonLondonUK

Section editors and affiliations

  • Elizabeth Hounsell
    • 1
  1. 1.School of Biological and Chemical SciencesBirkbeck College, University of LondonLondonUK